Chromatography quantitative measuring apparatus
View Patent ↗In a chromatography quantitative measuring apparatus according to the present invention, a beam applied from a light source to a chromatography test strip is formed into an elliptical shape by an optical means such as a cylindrical lens, a variation in absorbance that accompanies elution of a marker regent is detected while the elliptical beam is applied between a marker reagent hold part and a detection part, and a measurement is automatically started in a prescribed period of time since the detection of variation. According to the chromatography quantitative measuring apparatus so configured, non-uniform coloration is reduced by shaping the beam elliptically with the optical means, whereby the accuracy of quantitative analysis is enhanced, and the apparatus can be operated easily.
1. A method of chromatography quantitative measuring and determining a deficiency in an amount of inspection target solution and insufficient development of a chromatography test strip, said method comprising:
applying the inspection target solution to the chromatography test strip;
holding a marking reagent with a marker reagent hold part, the marker reagent capable of being eluted by development of the inspection target solution;
causing, in a base part, a specific binding reaction between the marker reagent and an analysis target included in the inspection target solution while the marker reagent and the analysis target are not immobilized; and
in a detection part, where a bounded material of the marker reagent and the analysis target is immobilized, using a chromatography quantitative measuring apparatus to apply an optical beam emitted from a light source to the chromatography test strip, detecting an optical signal utilizing a transmitted light or reflected light from the chromatography test strip, and quantitatively measuring the concentration of the analysis target included in the inspection target solution from the signal,
wherein during said quantitatively measuring the concentration of the analysis target, a position on the base part to which the marker reagent flows out of the inspection target solution is measured from the signal by applying the optical beam to a downstream end part of the base part on the chromatography test strip, thereby determining deficiency in the amount of inspection target solution applied and insufficient development on the chromatography test strip.
2. The method of claim 1 , wherein the optical beam is scanned from an upstream end part of the base part on the chromatography test strip to the downstream end part thereof.