Biocompatible phase invertable proteinaceous compositions and methods for making and using the same
View Patent ↗Biocompatible phase invertible proteinaceous compositions and methods for making and using the same are provided. The subject phase invertible compositions are prepared by combining a proteinaceous substrate and a crosslinking agent, such as a stabilized aldehyde crosslinking agent. Also provided are kits for use in preparing the subject compositions. The subject compositions, kits and systems find use in a variety of different applications.
1. A method of sealing a body site of a subject in need thereof, comprising the step of applying a composition comprising a stabilized dialdehyde crosslinker and a proteinaceous substrate to said site, wherein the dialdehyde has been stabilized by heating it to a temperature ranging from 40 to 60° C. for a period of time ranging from 1 to 20 days.
2. The method according to claim 1 , wherein the dialdehyde is heat treated glutaraldehyde.
3. The method according to claim 1 , wherein the composition comprises molecules described by the following formula:
wherein R1 and R2 are H or hydrocarbon moieties which are polymerization products produced by sequential aldol reaction and dehydration of one or more glutaraldehyde molecules.
4. The method according to claim 1 , wherein the site is an arterial vessel.
5. The method according to claim 1 , wherein the site is an organ.
6. The method according to claim 1 , wherein the site comprises connective tissue.
7. The method according to claim 6 , wherein the connective tissue comprises tissue selected from the group consisting of ligaments, tendons and bone.
8. The method according to claim 1 , wherein the subject is a mammal.
9. The method according to claim 8 , wherein the mammal is a human.
10. The method according to claim 1 , wherein the mammal is from an ungulate.
11. The method according to claim 10 , wherein the ungulate is selected from the group consisting of cows, pigs, sheep and horses.
12. The method according to claim 1 , wherein said composition is at an amount of from 1 to 100 ml.
13. A method of sealing an arterial vessel of a subject in need thereof, comprising the step of applying a composition comprising a stabilized dialdehyde crosslinker and a proteinaceous substrate to said arterial vessel, wherein the dialdehyde has been stabilized by heating it to a temperature ranging from 40 to 60° C. for a period of time ranging from 1 to 20 days.
14. The method according to claim 13 , wherein the dialdehyde is heat treated glutaraldehyde.
15. The method according to claim 13 , wherein the composition comprises molecules described by the following formula:
wherein R1 and R2 are H or hydrocarbon moieties which are polymerization products produced by sequential aldol reaction and dehydration of one or more glutaraldehyde molecules.
16. The method according to claim 13 , wherein said composition is at an amount of from 1 to 100 ml.
17. A method of sealing mammalian tissue of a subject in need thereof, comprising the step of applying a composition comprising a stabilized dialdehyde crosslinker and a proteinaceous substrate onto said mammalian tissue, wherein the dialdehyde has been stabilized by heating it to a temperature ranging from 40 to 60° C. for a period of time ranging from 1 to 20 days.
18. The method according to claim 17 , wherein the dialdehyde is heat treated glutaraldehyde.
19. The method according to claim 17 , wherein the composition comprises molecules described by the following formula:
wherein R1 and R2 are H or hydrocarbon moieties which are polymerization products produced by sequential aldol reaction and dehydration of one or more glutaraldehyde molecules.
20. The method according to claim 17 , wherein said composition is at an amount of from 1 to 100 ml.
21. The method according to claim 17 , wherein the tissue is an organ.
22. The method according to claim 17 , wherein the mammal is a human.
23. The method according to claim 17 , wherein the mammal is an ungulate.
24. The method according to claim 23 , wherein the ungulate is selected from the group consisting of cows, pigs, sheep and horses.