DNA polymerase fusions and uses thereof

1. A method for DNA synthesis at a pH from 9.5 to 12, comprising:

(a) providing a DNA polymerase fusion comprising a chimeric archaeal DNA polymerase fused to an Sso7D protein, wherein the chimeric archaeal DNA polymerase comprises a first amino acid sequence derived from a first polymerase species and a second amino acid sequence derived from a second polymerase species

and wherein the first amino acid sequence or the second amino acid sequence is derived from an archaeal polymerase;

(b) contacting said fusion with a nucleic acid template, wherein said fusion has enhanced DNA polymerase activity at a pH from 9.5 to 12 as compared to at a pH lower than 9.5 and permits DNA synthesis, and

(c) synthesizing said DNA from said nucleic acid template.

2. The method of claim 1 , further comprising contacting a PCR enhancing factor and/or an additive with said DNA polymerase fusion and said nucleic acid template.

3. A kit for performing at a pH from 9.5 to 12 a method selected from the group consisting of DNA synthesis, cloning of a DNA synthesis product, sequencing DNA, RT PCR, and linear or exponential PCR amplification, said kit comprising:

a DNA polymerase fusion comprising a chimeric archaeal DNA polymerase fused to an Sso7D protein, wherein the chimeric archaeal DNA polymerase comprises a first amino acid sequence derived from a first polymerase species and a second amino acid sequence derived from a second polymerase species and has enhanced DNA polymerase activity at a pH from 9.5 to 12 as compared to at a pH lower than 9.5 and wherein the first amino acid sequence or the second amino acid sequence is derived from an archaeal polymerase;

a buffer with a pH from 9.5 to 12; and

packaging materials therefor.

4. The kit of claim 3 , further comprising a PCR enhancing factor and/or an additive.

5. A composition for any one of DNA synthesis, cloning of a DNA synthesis product, sequencing DNA, linear or exponential PCR amplification, and RT-PCR, said composition comprising:

a DNA polymerase fusion and a buffer with a pH from 9.5 to 12, wherein the DNA polymerase fusion comprises a chimeric archaeal DNA polymerase fused to an Sso7D protein, wherein the chimeric archaeal DNA polymerase comprises a first amino acid sequence derived from a first polymerase species and a second amino acid sequence derived from a second polymerase species and has enhanced DNA polymerase activity at a pH from 9.5 to 12 as compared to at a pH lower than 9.5 and wherein the first amino acid sequence or the second amino acid sequence is derived from an archaeal polymerase.

6. The composition of claim 5 , wherein the buffer is a DNA synthesis buffer, a DNA cloning buffer a DNA sequencing buffer, a DNA sequencing buffer, or a PCR reaction buffer.

7. The composition of claim 5 , further comprising a PCR enhancing factor and/or an additive.

8. The method of claim 1 , wherein both the first polymerase species and the second polymerase species are derived from archaeal DNA polymerases.

9. The method of claim 1 , wherein the first polymerase species or the second polymerase species is selected from the group consisting of Pfu, KOD, Pfx, Vent, Deep Vent, Tgo, Pwo, and Pyrococcus furiosus DP1/DP2.

10. The kit of claim 3 , wherein both the first polymerase species and the second polymerase species are derived from archaeal DNA polymerases.

11. The kit of claim 3 , wherein the first polymerase species or the second polymerase species is selected from the group consisting of Pfu, KOD, Pfx, Vent, Deep Vent, Tgo, Pwo, and Pyrococcus furiosus DP1/DP2.

12. The composition of claim 5 , wherein both the first polymerase species and the second polymerase species are derived from archaeal DNA polymerases.

13. The composition of claim 5 , wherein the first polymerase species or the second polymerase species is selected from the group consisting of Pfu, KOD, Pfx, Vent, Deep Vent, Tgo, Pwo, and Pyrococcus furiosus DP1/DP2.