Hot spring bacterial strain BKH1 and protein isolated therefrom, concrete compositions, and uses thereof
View Patent ↗Disclosed is a Thermoanaerobacter sp. bacterial strain (BKH1) isolated from a hot spring, a purified protein (bioremediase) isolated from bacterial strain BKH1, as well as concrete compositions comprising BKH1 and/or the protein, and methods of using the protein and/or composition. Also disclosed are nucleic acids encoding the protein isolated from BKH1, as well as expression vectors, host cells, cell lines, and methods for generating and purifying the bioremediase protein.
1. A concrete composition comprising a cement matrix and an isolated protein comprising the amino acid sequence of SEQ ID NO:1, wherein the protein has silica releasing activity of about 70 μg silica/mg protein.
2. The concrete composition of claim 1 , wherein the amount of protein is effective to increase the strength of the concrete composition by about 25% relative to the strength of the concrete composition in the absence of the protein.
3. The concrete composition of claim 1 , wherein the amount of protein is from about 0.05 μg protein/g cement matrix to about 5.0 μg protein/g cement matrix.
4. The composition of claim 1 , wherein the protein is thermostable from about 50° C. to about 80° C.