Rebaudioside a composition and method for purifying rebaudioside a
View Patent ↗Exemplary embodiments of this invention encompass a method for purifying a low purity steviol glycoside composition. In particular, this invention relates to a method for purifying low purity steviol glycoside compositions to obtain substantially pure steviol glycoside compositions comprising approximately 95% or greater steviol glycosides and approximately 75% or greater rebaudioside A with a single crystallization step.
1. A method for purifying a low purity rebaudioside A composition comprising the steps of:
combining a low purity rebaudioside A composition with an aqueous organic solvent to form a low purity rebaudioside A solution, the low purity rebaudioside A composition containing rebaudioside A in a purity from 40% to 60% by weight on a dry basis, the aqueous organic solvent comprising water, in an amount from 5% to 10% water by weight, and ethanol;
heating the low purity rebaudioside A solution at a temperature in a range from 35° C. to 40° C.;
optionally seeding the rebaudioside A solution at an appropriate temperature with an amount of rebaudioside A sufficient to promote crystallization of rebaudioside A;
crystallizing from the low purity rebaudioside A solution in a single step a substantially pure steviol glycoside composition comprising rebaudioside A and one or more other steviol glycosides selected from the group consisting of rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, rebaudioside F, stevioside, dulcoside A, rubusoside, and steviolbioside; and
separating and washing the substantially pure steviol glycoside composition;
wherein the rebaudioside A is present in the substantially pure steviol glycoside composition in an amount greater than 95% by weight on a dry basis, and
wherein the mass yield of the rebaudioside A is greater than 20% by weight and the mass yield of the steviol glycosides is greater than 25% by weight.
2. The method of claim 1 , wherein rebaudioside A solution in the single crystallization step is stirred or unstirred.
3. The method of claim 1 , wherein the rebaudioside A solution is heated for a period of time from about 5 to about 60 minutes.
4. The method of claim 1 , wherein the crystallization of the steviol glycosides is allowed to continue for a period of time from about 0.5 hours to about 120 hours.
5. The method of claim 1 , wherein the aqueous organic solvent and the crude rebaudioside A are present in the rebaudioside A solution in a weight ratio from about 4 to about 10 parts aqueous organic solvent to about 1 part crude rebaudioside A.
6. The method of claim 1 , wherein the aqueous organic solvent further comprises a solvent selected from the group consisting of acetone, acetonitrile, methanol, 1-propanol, isopropanol, 1-butanol, 2-butanol, tert-butanol, and mixtures thereof.
7. The method of claim 1 , wherein the aqueous organic solvent further comprises methanol.
8. The method of claim 7 , wherein the ethanol and methanol are present in a weight ratio from about 20 parts to about 1 part ethanol to about 1 part methanol.
9. The method of claim 7 , wherein the ethanol and methanol are present in a weight ratio from about 3 parts to about 1 part ethanol to about 1 part methanol.
10. The method of claim 1 , further comprising cooling the rebaudioside A solution after the heating step.
11. The method of claim 1 , wherein the rebaudioside A solution is seeded with an amount of rebaudioside A sufficient to promote crystallization of rebaudioside A.
12. The method of claim 1 , further comprising drying the substantially pure steviol glycoside composition after washing the substantially pure steviol glycoside composition.
13. The method of claim 1 , wherein the substantially pure mixture of steviol glycosides comprises no more than 2% of steviolbioside.