Microfluidized oil-in-water emulsions and vaccine compositions
View Patent ↗This invention provides submicron oil-in-water emulsions useful as a vaccine adjuvant for enhancing the immunogenicity of antigens. The present invention also provides vaccine compositions containing an antigen combined with such emulsions intrinsically or extrinsically. Methods of preparing the emulsions and vaccines are also provided by the present invention.
1. A method of preparing a submicron oil-in-water emulsion, comprising:
(a) preparing a mixture by combining a light hydrocarbon non-metabolizable oil, one or more surfactants, and an aqueous component;
(b) subjecting said mixture to a primary emulsification process to produce an oil-in-water emulsion; and
(c) subjecting the oil-in-water emulsion prepared in (b) to microfluidization to produce said submicron oil-in-water emulsion, wherein the submicron emulsion has a mean oil droplet size of less than 1 μm, and wherein the distribution of droplet size is consistently from about 0.03 μm to about 1.0 μm.
2. The method of claim 1 , wherein said oil is in an amount of 1% to 50% v/v, and said surfactant is in an amount of 0.01% to 10% v/v.
3. The method of claim 2 wherein said mean oil droplet size in said submicron oil-in-water emulsion is less than 0.8 μm.
4. The method of claim 3 wherein said mean oil droplet size in said submicron oil-in-water emulsion is between 0.1-0.5 μm.
5. The method of claim 1 wherein said light hydrocarbon non-metabolizable oil is light mineral oil.
6. The method of claim 1 wherein said surfactant comprises a phospholipid compound or a mixture of phospholipid compounds.
7. The method of claim 6 wherein said phospholipid is selected from the group consisting of phosphatidylcholine, phosphatidylethanolmine, phosphatidylserine, phosphatidylethanolmine, phosphatidylinositol, phosphatidylglycerol, phosphatidic acid, spingomyelin and cardiolipin.
8. The method of claim 6 wherein said mixture of phospholipid compounds is lecithin.
9. The method of claim 1 wherein said surfactant comprises at least one of polyoxyethylene sorbitan mono-oleate or sorbitan mono-oleate.
10. The method of claim 1 wherein said microfluidization is conducted in a microfluidizer at an operating pressure in the range of about 1,000 to 15,000 psi.
11. The method of claim 1 , wherein the mixture formed in step (a) further includes an immunostimulatory molecule selected from Quil-A, GP-100, cholesterol or DDA.
12. The method of claim 1 , wherein the emulsion is stable for up to 24 months.