IP Library Granted Patent US 9,080,138
Granted Patent B2
US 9,080,138 · App. 12/665,020 · Granted Jul 14, 2015

Cell culture system, cell culture method, cell culture vessel and method for manufacturing cell culture vessel

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Quick Facts
Patent No.
US 9,080,138
App. No.
12/665,020
Granted
Jul 14, 2015
Kind
B2
Abstract

A cell culture system comprising a light source for emitting light, a light intensity regulator for regulating the light intensity of the light emitted by the light source, a cell activity-measuring device for measuring the activity of cells cultured on a photocatalytic film irradiated with the light, and an association device for associating the light intensity with the cell activity.

Claims (12)

1. A cell culture method comprising:

emitting light of which light intensity can be regulated;

preparing a cell incubator comprising a photocatalytic film consisting of titanium oxide and in which: a culture medium is injected; titanium in the titanium oxide reacts with water in the culture medium, by irradiated light, to form hydroxyl groups on the surface of the photocatalytic film; and the degree of hydrophilicity of the photocatalytic film surface varies depending on the light intensity of the light irradiated;

culturing cells on a surface of the photocatalytic film consisting of titanium oxide whose degree of hydrophilicity varies depending on the light intensity;

measuring the activity of cells cultured on the photocatalytic film irradiated with the light; and

associating the light intensity with the activity of the cells.

2. The cell culture method of claim 1 , further comprising extracting a value of the light intensity that maximizes the activity of the cells.

3. The cell culture method of claim 2 , further comprising irradiating the photocatalytic film with light having the light intensity of the extracted value and culturing the cells on a surface of the photocatalytic film.

4. The cell culture method of claim 1 , wherein the proliferation rate of the cells is measured in measuring the activity of the cells.

5. The cell culture method of claim 1 , wherein the growth rate of the cells is measured in measuring the activity of the cells.

6. The cell culture method of claim 1 , wherein the amount of secreted material indicative of the activity of the cells is measured in measuring the activity of the cells.

7. The cell culture method of claim 1 , wherein the gene expression level indicative of the activity of the cells is measured in measuring the activity of the cells.

Assignments (3)
RELEASE OF SECURITY INTEREST IN PATENTS, RECORDED ON JANUARY 29, 2019 AT REEL 048373 FRAME 0217 Recorded Sep 22, 2025
From: CRESTLINE DIRECT FINANCE, L.P., AS COLLATERAL AGENT
To: EMPIRE TECHNOLOGY DEVELOPMENT LLC
Reel/Frame 072936/0464 →
SECURITY INTEREST Recorded Jan 29, 2019
From: EMPIRE TECHNOLOGY DEVELOPMENT LLC
To: CRESTLINE DIRECT FINANCE, L.P.
Reel/Frame 048373/0217 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 21, 2010
From: KUSUURA, TAKAHISA
To: EMPIRE TECHNOLOGY DEVELOPMENT LLC
Reel/Frame 024721/0066 →