N-terminal modified exendin-4
The present invention relates to an N-terminal amino acid-modified insulinotropic peptide having a high activity, and to a pharmaceutical composition comprising the same. The insulinotropic peptide derivatives according to the present invention exhibit therapeutic effects, which are not observed in native and other insulinotropic peptide analogs. Therefore, the insulinotropic peptide derivatives and the pharmaceutical composition comprising the same according to the present invention can be effectively provided for the treatment of the diseases.
1. A modified exendin-4, wherein the N-terminal histidine residue of a native exendin-4 is substituted with a residue selected from the group consisting of N-dimethyl-histidyl, beta-hydroxy imidazopropionyl, 4-imidazoacetyl and beta-carboxy imidazopropionyl.
2. The modified exendin-4 as set forth in claim 1 , wherein the modified exendin-4 consists of an amino acid sequence of the following Formula 1:
R1-X—R2
wherein R1 is selected from the group consisting of N-dimethyl-histidyl, beta-hydroxy imidazopropionyl, 4-imidazoacetyl and beta-carboxy imidazopropionyl;
R2 is selected from the group consisting of —NH 2 or —OH;
X is Gly-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Y-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-Ile-Glu-Trp-Leu-Z-Asn-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser or Gly-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Y-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-Ile-Glu-Trp-Leu-Z-Asn-Gly-Gly;
Y is selected from the group consisting of Lys, Ser, and Arg; and
Z is selected from the group consisting of Lys, Ser, and Arg.
3. The modified exendin-4 as set forth in claim 2 , wherein R1 is N-dimethyl-histidyl, Y is Lys or Ser, Z is Lys, and R2 is —NH 2 .
4. The modified exendin-4 as set forth in claim 2 , wherein R1 is 4-imidazoacetyl, Y is Lys or Ser, Z is Lys, and R2 is —NH 2 .
5. The modified exendin-4 as set forth in claim 2 , wherein R1 is beta-hydroxy imidazopropionyl, Y is Lys or Ser, Z is Lys, and R2 is —NH 2 .
6. The modified exendin-4 as set forth in claim 2 , wherein R1 is beta-carboxy imidazopropionyl, Y is Lys or Ser, Z is Lys, and R2 is —NH 2 .
7. A pharmaceutical composition comprising the modified exendin-4 of claim 1 and a pharmaceutically acceptable carrier.
8. A pharmaceutical composition comprising the modified exendin-4 of claim 2 and a pharmaceutically acceptable carrier.