Test method on feline vaccinated with feline immunodeficiency virus vaccine, and antigen for use in the test
Since FIV-vaccinated cats produce antibodies against FIV, distinguishing them from FIV-infected cats is difficult by serological diagnostic methods using FIV and FIV-derived substances. The present invention enables tests for determining the presence or absence of a FIV vaccination history in a cat by detecting antibodies that are produced as a result of vaccination of a cat with an FIV vaccine, but not as a result of FIV infection. Using the methods of the present invention, whether an anti-FIV antibody-positive cat is infected with FIV or has been vaccinated can be conveniently distinguished.
1. A method for testing the presence or absence of a feline immunodeficiency virus (FIV) vaccination history in a cat, the method comprising detecting an antibody against a non-FIV-derived cellular component in a test sample from the cat using an antigen-antibody reaction, wherein the antibody is produced as a result of vaccination with an FIV vaccine but not as a result of FIV infection and the cat is determined to have an FIV vaccination history when the antibody is detected.
2. The test method of claim 1 , wherein the non-FIV-derived cellular component is a cell membrane, cytoplasm, nucleus, or cytoskeleton of an FIV-infected cell utilized to produce the FIV vaccine.
3. The test method of claim 1 , wherein the non-FIV-derived cellular component is a cellular component of a feline-derived cell.
4. The test method of claim 1 , wherein the non-FIV-derived cellular component is a cellular component of a cell derived from a non-feline animal.
5. The test method of claim 1 , wherein serum, plasma, or whole blood derived from the cat is used as the test sample.
6. The test method of claim 2 , wherein the non-FIV-derived cellular component is a cellular component of a feline-derived cell.
7. The test method of claim 2 , wherein the non-FIV-derived cellular component is a cellular component of a cell derived from a non-feline animal.
8. The test method of claim 2 , wherein serum, plasma, or whole blood derived from the cat is used as the test sample.
9. The test method of claim 3 , wherein serum, plasma, or whole blood derived from the cat is used as the test sample.
10. The test method of claim 4 , wherein serum, plasma, or whole blood derived from the cat is used as the test sample.