Ultraconserved regions encoding ncRNAs
View Patent ↗Described herein are methods for differentiate human cancers comprising using one or more transcribed ultraconserved regions (T-UCR) expression profiles where the association between the genomic location of UCRs and the analyzed cancer-related genomic elements is highly statistically significant and comparable to that reported for miRNAs.
1. A method to identify a relative risk in a vertebrate for developing chronic lymphocytic leukemia, comprising:
(a) obtaining a nucleic acid-containing test sample from a vertebrate subject,
(b) measuring, by laboratory analysis, a level of at least one transcribed ultra-conserved regions (T-UCR),
(c) identifying whether the test sample expresses at least one T-UCR,
(d) determining the relative risk for developing chronic lymphocytic leukemia by comparing the at least one T-UCR measured level to the level of a corresponding T-UCR in a control sample,
wherein a decrease in the level of at least one T-UCR in the test sample, relative to the level of a corresponding T-UCR in a control sample, is indicative of the subject either having, or being at risk for developing chronic lymphocytic leukemia, wherein the at least one T-UCR is uc.160(N), and
(e) measuring the level of at least one miR-155 in the test sample from said subject,
wherein an increase in the level of the miR-155 in the test sample, relative to the level of a corresponding miR-155 in a control sample, is indicative of the subject either having, or being at risk for developing chronic lymphocytic leukemia.
2. The method of claim 1 , further comprising measuring one or more additional T-URCs selected from: uc.269A(N) and uc.346A(P).
3. The method of claim 1 , further comprising measuring at least one additional T-UCR selected from uc.215(N) and uc.348(N).