Gradient injection for biosensing
View Patent ↗A single injection gradient with a biosensor, both structural and methodological, achieves the binding of analyte to immobilized ligand over a wide concentration range without the necessity of regeneration of the sensing area. A gradient of concentrations adjacent to or within a flow cell facilitates kinetic analysis of interactions without requiring multiple discrete volumes or injections to achieve a range of concentrations. A continuous gradient fluid is preferably formed directly adjacent to the flow cell inlet or a region of sample/buffer dispersion at an injection point into a flow channel of a flow cell. The analyte gradient may be flowed through the flow cell from a low analyte concentration. Multiple component gradients are also provided.
1. A method for determining an affinity or kinetic constant for ligand binding comprising the steps of:
creating a gradient sample by mixing a first gradient component solution comprising an analyte having a starting concentration with a second gradient component solution in a channel connected to an inlet of a flow cell such that an analyte concentration gradient is generated thereby providing a range of analyte concentrations within the gradient sample;
injecting, in a single, continuous injection, the gradient sample into the flow cell and onto a first sensing surface and a second sensing surface, wherein the first sensing surface comprises a ligand immobilized thereon and the second sensing surface is free of immobilized ligand;
adding to one of the gradient component solutions a material that changes the bulk refractive index thereof;
recording a first response at the first sensing surface and a second response at the second sensing surface, wherein the first response includes a binding response component elicited by an interaction between the analyte and the ligand thereby providing a binding response curve, and wherein the second response provides an actual bulk refractive index response of the gradient sample;
determining a gradient injection profile based on the second response;
extrapolating the range of analyte concentrations present at the first sensing surface by performing the following steps:
generating an adjusted bulk refractive index response by dividing the actual bulk refractive index response by a maximum expected bulk refractive index response for a 100% pure solution of the material that changes the bulk refractive index;
fitting an equation to the adjusted bulk refractive index response using a regression curve fitting procedure that suitably models the gradient injection profile of the gradient sample as a function of injection time thereby defining a gradient injection profile function;
multiplying the starting concentration of the analyte by the gradient injection profile function in order to estimate the range of analyte concentrations at the first sensing surface as a function of time during the gradient injection;
using the extrapolated range of analyte concentrations for determining at least one of a plurality of kinetic or affinity constants for the binding response component.
2. The method of any one of claim 1 wherein the material that changes the bulk refractive index is selected from the group comprising sugars, polysaccharides, glycols, hydrogels, salts, hydrophilic polymers and water miscible solvents.
3. The method of claim 1 wherein the first response further comprises a bulk refractive index component such that individual response contributions of the binding response component and the bulk refractive index component are resolved from the binding response curve as a function of injection time by employing non-linear regression curve fitting of predefined mathematical models.