Nucleic acid detection method and nucleic acid detection kit

1. A nucleic acid detection method for detecting an amplified double-stranded DNA fragment amplified by a nucleic acid amplification method, wherein:

the amplified DNA fragment has a first substance binding site to which the first substance is specifically bindable; and

the nucleic acid detection method comprises the step of:

concentrating the amplified DNA fragment by causing the amplified DNA fragment to bind to the first substance;

wherein the amplified DNA fragment further has a second substance binding site to which the second substance is specifically bindable; and

said step of concentrating including:

(i) forming a complex by binding the amplified DNA fragment to the second substance to which a labeling substance is bound, and

(ii) binding the complex to the first substance; and

wherein the amplified DNA fragment is a double-stranded DNA fragment obtained by the nucleic acid amplification method by use of two primers;

one of the two primers has a base sequence to which the first substance is bindable in a double strand formed by binding the one of the two primers to a complementary strand; and

the other one of the two primers has a base sequence to which the second substance is bindable in a double strand formed by binding the other one of the two primers to a complementary strand; and

said step of concentrating further including:

(i) on a stationary-phase medium to which the first substance is immobilized, placing the amplified DNA fragment in a first region, and placing the second substance to which the labeling substance is bound in a third region, the first region and third region being different from each other and being different from a second region in which the first substance is immobilized,

(ii) diffusing the amplified DNA fragment by use of a solvent to the third region,

(iii) forming a complex in the third region by binding the amplified DNA fragment to the second substance to which the labeling substance is bound,

(iv) diffusing the complex on the stationary-phase medium to the second region by use of the solvent, and

(v) binding the first substance and the complex to each other in the second region; and

wherein the stationary-phase medium further has a fourth region where a nucleic acid fragment that can specifically bind to the second substance can be immobilized; and

the second substance to which the labeling substance is bound and which is diffused from a region for placing the second substance to which the labeling substance is bound binds to the nucleic acid fragment in the fourth region; and

wherein the first substance and the second substance are each a methylase.

2. The nucleic acid detection method as set forth in claim 1 , wherein:

the first substance and the second substance are different from each other.