MHC multimers in tuberculosis diagnostics, vaccine and therapeutics
The present invention relates to MHC-peptide complexes and uses thereof in the diagnosis of, treatment of or vaccination against a disease in an individual. More specifically the invention discloses MHC complexes comprising Mycobacterium tuberculosis antigenic peptides and uses there of.
1. A composition comprising a plurality of soluble MHC multimers wherein, each MHC multimer comprises (a-b-P)n,
wherein n>4,
wherein a and b together form a functional MHC protein capable of binding the peptides P,
wherein (a-b-P) is the MHC-peptide complex formed when the peptide P binds to the functional MHC protein,
wherein each MHC-peptide complex of each MHC multimer is associated with one or more multimerization domains selected from the group consisting of polysaccharides, dextran moieties, coiled-coil polypeptide structures, avidins, streptavidins and antibodies, wherein each MHC protein is an HLA-A*0201 allele, and
wherein the composition comprises at least one multimer wherein each P is SEQ ID NO: 12637, at least one multimer wherein each P is SEQ ID NO: 12603, at least one multimer wherein each P is SEQ ID NO: 12731, at least one multimer wherein each P is SEQ ID NO: 124191, at least one multimer wherein each P is SEQ ID NO: 124224, at least one multimer wherein each P is SEQ ID NO: 124174 and at least one multimer wherein each P is SEQ ID NO: 124187.
2. A kit comprising the composition according to claim 1 .
3. The kit according to claim 2 , comprising at least one additional component.
4. The kit according to claim 3 , wherein the additional component is a positive control.
5. The kit according to claim 3 , wherein the additional component is instructions for use.
6. The composition according to claim 1 , wherein the MHC multimer comprises one or more covalently or non-covalently attached label selected from the group consisting of fluorophores, fluorescent labels, enzyme labels, radioactive labels, chemiluminescent labels, dyes, chromophores, fluorochromes, bioluminescent labels, metal particles, haptens, DNA fluorescing stains, polymers, and antibodies.
7. The composition according to claim 1 , wherein n>10.
8. The composition according to claim 1 , wherein n is between 10 and 20.