Phosphonate fluorescent dyes and conjugates
Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
1. A compound having the formula:
wherein
L is a member selected from the group consisting of a (C 1 -C 20 )alkylene and (C 1 -C 20 ) heteroalkylene linking group;
X is a member selected from the group consisting of a functional group and a protected functional group wherein said functional group is hydroxy, diol, carboxy, formyl, carbonyl, thio, aminooxy, azido, carbazoyl, hydrazine, or amino having the formula NR A R B ;
R is —(CH 2 ) n —NR A R B , wherein n is 3, 4, or 5; wherein
R A is H or (C 1 -C 8 )alkyl and R B is selected from the group consisting of —C(O)CF 3 , —C(O)OtBu, FMOC, monomethoxytrityl and —C(O)O-benzyl.
2. A compound of claim 1 , wherein X is —NR A R B .
3. A compound of claim 1 , wherein X is —O-dimethyoxytrityl.
4. A compound of claim 1 , selected from the group consisting of