Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
View Patent ↗A signal detection system configured for detecting a signal emitted by the contents of a reaction receptacle is also configured to apply thermal energy to a portion of the reaction receptacle to affect a reaction occurring within the reaction receptacle. More particularly, a system for detecting electromagnetic radiation emitted by the contents of a reaction receptacle includes a transmission element configured for transmitting electromagnetic radiation from the contents of the receptacle, a thermal element associated with the transmission element and configured to apply thermal energy to at least a portion of the receptacle, and a detector configured to receive electromagnetic radiation from the transmission element and to generate a signal corresponding to a characteristic of the electromagnetic radiation received by the detector.
1. A system for detecting electromagnetic radiation from the contents of a receptacle, said system comprising:
a receptacle holding area configured to hold a receptacle;
a receptacle held in said receptacle holding area;
a detection element configured to receive electromagnetic radiation and further adapted to generate a signal corresponding to a characteristic of the electromagnetic radiation received by the detection element;
a transmission element disposed between said receptacle held in said receptacle holding area and said detection element and adapted to transmit electromagnetic radiation from the contents of the receptacle to the detection element;
a thermal element in thermal conductivity with at least a portion of said transmission element and constructed and arranged to apply thermal energy to at least a portion of said transmission element; and
an actuator mechanism constructed and arranged to move said transmission element between first and second positions, wherein said transmission element is in greater contact with said receptacle in the second position than in the first position,
wherein said receptacle comprises a compressible portion that is at least partially compressed by said transmission element when said actuator moves said transmission element from the first position to the second position.
2. The system of claim 1 , wherein said detection element comprises a photodiode.
3. The system of claim 1 , further comprising an emission element adapted to emit electromagnetic radiation.
4. The system of claim 3 , wherein said emission element comprises a light-emitting diode.
5. The system of claim 1 , wherein at least a portion of said transmission element is configured to be in contact with at least a portion of the receptacle.
6. The system of claim 1 , wherein said transmission element comprises an optic element adapted to transmit a light emission from the contents of the receptacle.
7. The system of claim 6 , wherein said optic element is a transparent or translucent material.
8. The system of claim 7 , wherein said optic element is a plastic.
9. The system of claim 6 , wherein said optic element is adapted to transmit fluorescent light through at least a portion of said optic element.
10. The system of claim 1 , wherein said thermal element comprises an electrically-resistive film secured to a surface of said transmission element or embedded in at least a portion of said transmission element, said electrically-resistive film having an opening therein through which the electromagnetic radiation can be transmitted.
11. The system of claim 1 , wherein said transmission element comprises a peripheral wall defining a cavity, and wherein said thermal element comprises an electrically-resistive film secured to a surface of said peripheral wall or embedded in at least a portion of said peripheral wall for applying thermal energy to the space within said cavity.
12. The system of claim 1 , wherein said thermal element comprises an electrically-resistive film secured to a surface of said transmission element or embedded in at least a portion of said transmission element, said electrically-resistive film being adapted to transmit the electromagnetic radiation through the resistive film.
13. The system of claim 1 , wherein the electromagnetic radiation is fluorescence and said detection element is adapted to generate a signal corresponding to the intensity of the fluorescence.
14. The system of claim 1 , wherein said transmission element is not in contact with said receptacle in the first position.
15. The system of claim 1 , wherein said compressible portion comprises a chamber formed from a flexible material configured to yield to pressure applied externally to at least a portion of said chamber.
16. The system of claim 1 , further comprising a thermal element disposed adjacent to the receptacle holding area in opposed relationship to said transmission element.
17. A method for detecting electromagnetic radiation from the contents of a receptacle, said method comprising:
transmitting electromagnetic radiation from the contents of the receptacle to a detection element with a transmission element disposed between the receptacle and the detection element;
applying thermal energy to the transmission element to cause a temperature of at least a portion of the transmission element to be different from ambient temperature;
detecting the electromagnetic radiation with the detection element;
moving the transmission element between first and second positions, wherein said transmission element is in greater contact with the receptacle in the second position than in the first position; and
compressing at least a portion of the receptacle with the transmission element when the transmission element is in the second position.
18. The method of claim 17 , wherein the step of applying thermal energy comprises heating a portion of the transmission element to a temperature above ambient temperature.
19. The method of claim 17 , further comprising causing the transmission element to be in contact with at least a portion of the receptacle.
20. The method of claim 17 , wherein the transmission element is not in contact with the receptacle in the first position.
21. The method of claim 17 , wherein the portion of the receptacle compressed with the transmission element comprises a chamber formed from a flexible material configured to yield to pressure applied externally to at least a portion of said chamber, so that compressing the chamber reduces an internal volume of the chamber.
22. The method of claim 17 , wherein the transmitting step comprises transmitting a light emission from the contents of the receptacle.
23. The method of claim 22 , wherein the detecting step comprises detecting a fluorescent emission front the contents of the receptacle.
24. The method of claim 23 , further comprising directing excitation energy of a prescribed wavelength at the contents of the receptacle.
25. The method of claim 17 , wherein the thermal energy is applied by a thermal element in thermal contact with the transmission element.
26. The method of claim 25 , wherein the thermal element is in thermal contact with a portion of the transmission element through which the electromagnetic radiation is transmitted to the detection element.
27. The method of claim 25 , wherein the thermal element is in thermal contact with a portion of a transmission element surrounding a portion of the transmission element through which the electromagnetic radiation is transmitted to the detection element.
28. The method of claim 25 , wherein the transmission element comprises a peripheral wall defining a cavity, and wherein the thermal element is in thermal contact with at least a portion of the peripheral wall.
29. The method of claim 25 , wherein the thermal element is secured to a surface of the transmission element or embedded in at least a portion of the transmission element.
30. The method of claim 17 , further comprising applying thermal energy to the receptacle with a thermal element disposed adjacent the receptacle and in opposed relationship to the transmission element.
31. The method of claim 17 , wherein detecting the electromagnetic radiation comprises detecting electromagnetic radiation of a prescribed wavelength.
32. The method of claim 17 , wherein detecting the electromagnetic radiation comprises detecting the intensity of the electromagnetic radiation.
33. The method of claim 17 , wherein the contents of the receptacle are maintained at a substantially uniform temperature.
34. The method of claim 33 , wherein the contents of the receptacle are maintained at an essentially constant temperature during said method.
35. The method of claim 17 , further comprising performing an amplification reaction in the receptacle during said method.
36. A device adapted to transmit electromagnetic radiation and to apply thermal energy to a receptacle, said device comprising:
a transmission element adapted to transmit a light emission through at least a portion of said transmission element;
a thermal element secured to a surface of said transmission element or embedded in at least a portion of said transmission element and constructed and arranged to apply thermal energy to at least a portion of said transmission element to thereby raise or lower an outer surface temperature of said transmission element; and
an actuator mechanism constructed and arranged to move said transmission element between first and second positions with respect to a receptacle disposed adjacent said transmission element, such that said transmission element will be in greater contact with the receptacle in the second position than in the first position, and whereby the receptacle will be at least partially compressed by said transmission element when said actuator moves said transmission element from the first position to the second position.
37. The device of claim 36 , wherein said transmission element is a transparent or translucent material.
38. The device of claim 37 , wherein said transmission element is a plastic.
39. The device of claim 36 , wherein said transmission element is adapted to transmit fluorescent light through at least a portion of said optic element.
40. The device of claim 36 , wherein said thermal element comprises an electrically-resistive film secured to a surface of said transmission element or embedded in at least a portion of said transmission element, said electrically-resistive film having an opening therein through which electromagnetic radiation can be transmitted.
41. The device of claim 36 , wherein said transmission element comprises a peripheral wall defining a cavity, and wherein said thermal element comprises an electrically-resistive film secured to a surface of said peripheral wall or embedded in at least a portion of said peripheral wall for applying thermal energy to the space within said cavity.
42. The device of claim 36 , wherein said thermal element comprises an electrically-resistive film secured to a surface of said transmission element or embedded in at least a portion of said transmission element, said electrically-resistive film being adapted to transmit electromagnetic radiation through the resistive film.