Lipolytic enzyme uses thereof in the food industry
View Patent ↗The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transfers an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Corynebacterium species.
1. A method of preparing a lysophospholipid comprising treating a substrate comprising a phospholipid with at least one lipolytic enzyme to produce said lysophospholipid, wherein said lipolytic enzyme has phospholipase activity and wherein said lipolytic enzyme is obtainable from the genus Thermobifida , wherein phospholipase activity is determined by an assay comprising mixing the enzyme with a pH 7 buffered substrate solution containing phosphatidylcholine at 37 degrees C. for 10 minutes, then raising the temperature to 99 degrees C. to stop any reaction, and analyzing any resultant free fatty acid, whereby if there is resultant free fatty acid, enzymatic activity is calculated as micromole fatty acid produced per minute at pH 7 and the enzyme has phospholipase activity,
wherein said lipolytic enzyme comprises at least one of a GDSx motif, or a GANDY block or a HPT block, and
wherein said lipolytic enzyme comprises an amino acid sequence shown as SEQ ID NO: 5 or an amino acid sequence having at least 90% identity therewith.
2. A method according to claim 1 , wherein said lipolytic enzyme comprises an amino acid sequence encoded by nucleotide sequence shown as SEQ ID NO: 6 or a nucleotide sequence which has at least 90% identity therewith.