System, method, and product for multiple wavelength detection using single source excitation
View Patent ↗An embodiment of a method for adjusting system gain of a biological probe array scanner for a plurality of fluorophore species is described that comprises setting an excitation beam comprising an excitation wavelength at a first power level that elicits an optimal signal to noise ratio response from a first fluorophore species; scanning a biological probe array with the excitation beam; setting the excitation beam comprising the excitation wavelength at a second power level different than the first power level that elicits the optimal signal to noise ratio response from a second fluorophore species; and scanning the biological probe array with the excitation beam.
1. A method of detecting multiple different wavelengths of light, which comprises:
providing an array comprising at least two fluorophores, wherein the at least two fluorophores emit different wavelengths of light upon excitation;
providing a scanner comprising an excitation beam and a color correcting lens, wherein the color correcting lens corrects chromatic aberrations which arise when the at least two different wavelengths of light pass through a lens;
scanning the array with the excitation beam, whereby the different wavelengths of light emitted from the fluorophores on the array exhibit chromatic aberrations;
directing the at least two different wavelengths of light emitted from the fluorophores on the array through the color correcting lens such that the at least two different wavelengths of light pass through a single focal point, thereby detecting multiple different wavelengths of light.
2. The method according to claim 1 , wherein chromatic aberrations and spherical aberrations are corrected by the lens.
3. The method according to claim 1 , wherein the color correcting lens comprises multiple lens elements to correct for differences associated with different indices of refraction of each fluorophore.
4. The method according to claim 3 , wherein all light that has passed through the multiple lens elements possess a single focal length.
5. The method according to claim 1 , wherein each fluorophore has associated therewith a different index of refraction.
6. The method according to 1 , wherein the color correcting lens further comprises a spatial component which corrects for differences in emitted light diameter size of each fluorophore.
7. The method according to claim 1 , wherein the at least two fluorophores comprises four fluorophores.
8. The method according to claim 7 , wherein the array is a biological probe array and wherein each of the four fluorophores corresponds to one of four nucleic acids.
9. The method according to claim 1 , wherein the array is a biological probe array.
10. The method according to claim 9 , wherein the biological probe array comprises DNA probes.
11. A system for scanning a plurality of fluorophores on an array, comprising:
an instrument control application stored for execution in system memory of a computer, wherein the application performs the method comprising:
providing an array comprising at least two fluorophores, wherein the at least two fluorophores emit different wavelengths of light upon excitation;
providing a scanner comprising an excitation beam and a color correcting lens, wherein the color correcting lens corrects chromatic aberrations which arise when two different wavelengths of light pass through a lens;
scanning the array with the excitation beam, whereby the different wavelengths of light emitted from the fluorophores on the array exhibit chromatic aberrations;
directing the at least two different wavelengths of light emitted from the fluorophores on the array through the color correcting lens such that the at least two different wavelengths of light pass through a single focal point, thereby detecting multiple different wavelengths of light.
12. The system according to claim 11 , wherein the at least two different wavelengths of light are selected from the group consisting of: 510, 520 and 530 nm.
13. The system according to claim 11 , wherein the color correcting lens comprises multiple lens elements to correct for differences associated with different indices of refraction of each fluorophore.
14. The system according to claim 13 , wherein each fluorophore has associated therewith a different index of refraction.
15. The system according to claim 11 , wherein all light that has passed through the multiple lens elements possess a single focal length.
16. The system according to 11 , wherein the color correcting lens further comprises a spatial component which corrects for differences in emitted light diameter size of each fluorophore.
17. The system according to claim 11 , wherein the at least two fluorophores comprises four fluorophores.
18. The system according to claim 17 , wherein the array is a biological probe array and wherein each of the four fluorophores corresponds to one of four nucleic acids.
19. The system according to claim 11 , wherein the array is a biological probe array.
20. The system according to claim 19 , wherein the biological probe array comprises DNA probes.