Disposable tubing set for use with a cell expansion apparatus and method for sterile sampling
View Patent ↗A disposable apparatus for cell expansion, having at least one bioreactor. The bioreactor has a cellular growth area and a supply area, the cellular growth area being separated from said supply area by a membrane. A fluid recirculation path in fluid communication with the cellular growth area allows for hermetically removing a sample containing cellular matter. This may comprise an elongated tube, or a plurality of parallel tube segments. The parallel tube segments have inflow ends and outflow ends, and the inflow ends are joined at a first common juncture and the outflow ends are joined at a second common juncture. The common junctures may comprise valves.
1. A method of expanding cellular matter, said method comprising providing at least one bioreactor, said bioreactor having a cellular growth area and a supply area, said cellular growth area being separated from said supply area by a membrane, said membrane being adapted to inhibit migration of cells from said cellular growth area to said supply area and to permit migration of certain chemical compounds from said cellular growth area to said supply area and of certain other chemical compounds from said supply area to said cellular growth area;
conducting a fluid containing cellular matter into said cellular growth area;
providing oxygenated fluid to said supply area to maintain conditions conducive for cell growth in said cellular growth area;
providing a plurality of parallel tube segments, said parallel tube segments having inflow ends and outflow ends, and said inflow ends are joined at a first common juncture comprising a valve and said outflow ends are joined at a second common juncture;
flowing fluid containing cellular matter through at least a first segment;
interrupting flow through said first segment while simultaneously flowing said fluid through a second segment; and
hermetically removing said first segment, said first segment containing a sample containing cellular matter from a fluid recirculation path in fluid communication with said cellular growth area.
2. The method of claim 1 further comprising providing an elongated tube, the length of said tube being long enough to permit at least one length containing said sample to be removed from said elongated tube;
placing a loop of said elongated tube in a sealing device such that said tube forms an intersection with itself in said sealing device;
severing said tube at said intersection to form two free ends of said tube and a looped tube segment having two ends;
cauterizing the two ends of said tube segment; and
sealing said two free ends of said tube together to re-connect said tube.
3. The method of claim 1 further comprising sampling non-cellular matter from said apparatus at least one sample port, said sample port being adapted to allow only non-cellular matter to be sampled.