IP Library Granted Patent US 8,323,495
Granted Patent B2
US 8,323,495 · App. 12/714,868 · Granted Dec 4, 2012

Method of operating a bioreactor and filtration system

View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 8,323,495
App. No.
12/714,868
Granted
Dec 4, 2012
Kind
B2
Abstract

The disclosure describes a novel method for operating a bioreactor and filtration system. The method adds excess iron to the bioreactor to improve the overall operation and efficiency of the bioreactor/filtration system.

Claims (39)

1. A method of operating a bioreactor and filtration system that reduces the fouling of the filtration system comprises:

feeding water containing contaminants into a bioreactor system containing live microorganisms, the bioreactor system comprising at least a first bioreactor;

feeding a concentration of iron into the first bioreactor above a stoichiometric requirement for iron in the first bioreactor; and

treating, directly or indirectly, effluent from the bioreactor system with a filtration system to obtain a filtration system effluent substantially free of the contaminants,

wherein the concentration of iron above the stoichiometric requirement fed into the first bioreactor results in a reduction in fouling of the filtration system when compared to utilizing the stoichiometric requirement for iron in the first bioreactor.

2. The method of claim 1 , wherein the contaminants are generated by at least one of a human, an animal, an industrial process, and a subsurface environment.

3. The method of claim 1 , wherein iron is added in a divalent form.

4. The method of claim 1 , wherein the microorganisms are bacteria and at least one of fungus, yeast, and protozoa.

5. The method of claim 1 , wherein the contaminants include methanol.

6. The method of claim 1 , further comprising:

feeding effluent from the first bioreactor into a second bioreactor containing live microorganisms; and

feeding a concentration of iron into the second bioreactor above the stoichiometric requirement for iron in the second bioreactor,

wherein the concentration of iron above the stoichiometric requirement fed into the second bioreactor results in a reduction in fouling of the filtration system when compared to utilizing the stoichiometric requirement for iron in the second bioreactor.

7. A method of operating a bioreactor and filtration system that reduces the fouling of the filtration system comprises:

feeding water containing contaminants into a bioreactor system containing live microorganisms, the bioreactor system comprising at least a first bioreactor;

feeding a concentration of iron into the first bioreactor from 150% to 10,000% above a stoichiometric requirement for iron in the first bioreactor; and

treating, directly or indirectly, effluent from the bioreactor system with a filtration system to obtain a filtration system effluent substantially free of the contaminants,

wherein the concentration of iron above the stoichiometric requirement fed into the first bioreactor results in a reduction in fouling of the filtration system when compared to utilizing the stoichiometric requirement for iron in the first bioreactor.

8. The method of claim 7 , wherein the step of feeding the concentration of iron into the bioreactor comprises utilizing from 150% to 6000% above the stoichiometric requirement for iron in the first bioreactor.

9. The method of claim 7 , wherein the step of feeding the concentration of iron into the bioreactor comprises utilizing from 200% to 500% above the stoichiometric requirement for iron in the first bioreactor.

10. The method of claim 7 , wherein the step of feeding the concentration of iron into the bioreactor comprises utilizing from 2000% to 4000% above the stoichiometric requirement for iron in the first bioreactor.

11. The method of claim 7 , wherein the step of feeding the concentration of iron into the bioreactor comprises utilizing from 3000% to 5000% above the stoichiometric requirement for iron in the first bioreactor.

12. The method of claim 7 , wherein the step of feeding the concentration of iron into the bioreactor comprises utilizing from 5000% to 10,000% above the stoichiometric requirement for iron in the first bioreactor.

13. The method of claim 7 , further comprising:

feeding effluent from the first bioreactor into a second bioreactor containing live microorganisms; and

feeding a concentration of iron into the second bioreactor from 150% to 10,000% above the stoichiometric requirement for iron in the first bioreactor,

wherein the concentration of iron above the stoichiometric requirement fed into the second bioreactor results in a reduction in fouling of the filtration system when compared to utilizing the stoichiometric requirement for iron in the first bioreactor.

14. The method of claim 7 , further comprising:

feeding effluent from the first bioreactor into a second bioreactor containing live microorganisms; and

feeding a concentration of iron into the second bioreactor from 200% to 500% above the stoichiometric requirement for iron in the first bioreactor,

wherein the concentration of iron above the stoichiometric requirement fed into the second bioreactor results in a reduction in fouling of the filtration system when compared to utilizing the stoichiometric requirement for iron in the first bioreactor.

15. The method of claim 7 , wherein the contaminants are generated by at least one of a human, an animal, an industrial process, and a subsurface environment.

16. The method of claim 7 , wherein iron is added in a divalent form.

17. The method of claim 7 , wherein the microorganisms are bacteria and at least one of fungus, yeast, and protozoa.

18. The method of claim 7 , wherein the contaminants include methanol.

19. The method of claim 7 , further comprising

feeding effluent from the first bioreactor into a second bioreactor containing live microorganisms; and

feeding a concentration of iron into the second bioreactor from 200% to 2000% above the stoichiometric requirement for iron in the first bioreactor,

wherein the concentration of iron above the stoichiometric requirement fed into the second bioreactor results in a reduction in fouling of the filtration system when compared to utilizing the stoichiometric requirement for iron in the first bioreactor.

Assignments (12)
SECURITY INTEREST Recorded Mar 12, 2026
From: NGL ENERGY PARTNERS LP; HILLSTONE ENVIRONMENTAL PARTNERS, LLC; ANTICLINE DISPOSAL, LLC; NGL WATER SOLUTIONS PERMIAN, LLC
To: BARCLAYS BANK PLC, AS COLLATERAL AGENT
Reel/Frame 074061/0417 →
SECURITY INTEREST Recorded Feb 6, 2024
From: NGL ENERGY PARTNERS LP; NGL ENERGY OPERATING LLC; HILLSTONE ENVIRONMENTAL PARTNERS, LLC; ANTICLINE DISPOSAL, LLC; NGL WATER SOLUTIONS PERMIAN, LLC
To: TORONTO DOMINION (TEXAS) LLC, AS ADMINISTRATIVE AGENT AND COLLATERAL AGENT
Reel/Frame 066391/0921 →
SECURITY INTEREST Recorded Feb 5, 2024
From: NGL ENERGY PARTNERS LP; HILLSTONE ENVIRONMENTAL PARTNERS, LLC; ANTICLINE DISPOSAL, LLC; NGL WATER SOLUTIONS PERMIAN, LLC
To: U.S. BANK TRUST COMPANY, NATIONAL ASSOCIATION
Reel/Frame 066385/0698 →
RELEASE OF SECURITY INTEREST Recorded Feb 5, 2024
From: U.S. BANK TRUST COMPANY, NATIONAL ASSOCIATION, IN ITS CAPACITY AS SUCCESSOR TO U.S. BANK NATIONAL ASSOCIATION
To: NGL ENERGY PARTNERS LP; NGL ENERGY OPERATING LLC; HILLSTONE ENVIRONMENTAL PARTNERS, LLC (AS SUCCESSOR-BY-MERGER TO HEP SHALEWATER SOLUTIONS, LLC)
Reel/Frame 066349/0743 →
PATENT SECURITY INTEREST AGENT AGREEMENT Recorded Jul 14, 2022
From: U.S. BANK NATIONAL ASSOCIATION, AS COLLATERAL AGENT
To: U.S. BANK TRUST COMPANY, NATIONAL ASSOCIATION, AS COLLATERAL AGENT
Reel/Frame 060650/0977 →
RELEASE OF SECURITY INTEREST Recorded Feb 18, 2021
From: DEUTSCHE BANK TRUST COMPANY AMERICAS
To: ANTICLINE DISPOSAL, LLC; NGL WATER SOLUTIONS PERMIAN, LLC
Reel/Frame 055325/0783 →
RELEASE OF SECURITY INTEREST Recorded Feb 18, 2021
From: DEUTSCHE BANK TRUST COMPANY AMERICAS
To: ANTICLINE DISPOSAL, LLC; NGL WATER SOLUTIONS PERMIAN, LLC
Reel/Frame 055325/0834 →
PATENT SECURITY AGREEMENT (BOND) Recorded Feb 4, 2021
From: NGL ENERGY OPERATING LLC; NGL ENERGY PARTNERS LP; HEP SHALEWATER SOLUTIONS, LLC; ANTICLINE DISPOSAL, LLC; NGL WATER SOLUTIONS PERMIAN, LLC
To: U.S BANK NATIONAL ASSOCIATION, AS COLLATERAL AGENT
Reel/Frame 055223/0741 →
PATENT SECURITY AGREEMENT (ABL) Recorded Feb 4, 2021
From: NGL ENERGY OPERATING LLC; NGL ENERGY PARTNERS LP; HEP SHALEWATER SOLUTIONS, LLC; ANTICLINE DISPOSAL, LLC; NGL WATER SOLUTIONS PERMIAN, LLC
To: JPMORGAN CHASE BANK, N.A,, AS COLLATERAL AGENT
Reel/Frame 055223/0725 →
SECURITY INTEREST Recorded Sep 6, 2019
From: ANTICLINE DISPOSAL, LLC; NGL WATER SOLUTIONS PERMIAN, LLC
To: DEUTSCHE BANK TRUST COMPANY AMERICAS
Reel/Frame 050297/0272 →
SECURITY AGREEMENT Recorded Jun 26, 2012
From: ANTICLINE DISPOSAL, LLC
To: DEUTSCHE BANK TRUST COMPANY AMERICAS, AS COLLATERAL AGENT
Reel/Frame 028442/0845 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 29, 2010
From: SHAFER, LEE L
To: ANTICLINE DISPOSAL, LLC
Reel/Frame 025064/0328 →