Method for continuously detecting glucose concentration in sample, kit thereof and method for using biosensor
The invention provides a method for continuously detecting glucose concentration in a sample, including: (a) providing a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer; (b) providing a carbohydrate binding protein solution, wherein the carbohydrate binding protein has at least one receptor, and the receptor is capable of binding to the polysaccharide and glucose; (c) mixing a sample and the carbohydrate binding protein solution to form a mixture; (d) contacting the mixture with the biosensor; (e) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor, wherein glucose concentration of the sample is inversely proportional to the amount of carbohydrate binding proteins bound to the polysaccharide; and (f) refreshing the surface of the biosensor with a high concentration glucose solution.
1. A method for continuously detecting glucose concentration in a sample, comprising:
(a) providing a biosensor comprising a transducer and a polysaccharide covered on a surface of the transducer;
(b) providing a carbohydrate binding protein solution, wherein the carbohydrate binding protein has at least one receptor, and the receptor is capable of binding to the polysaccharide and glucose;
(c) mixing a sample and the carbohydrate binding protein solution to form a mixture;
(d) contacting the mixture with the biosensor;
(e) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor, wherein glucose concentration of the sample is inversely proportional to the amount of carbohydrate binding proteins bound to the polysaccharide;
(f) refreshing the surface of the transducer with a high concentration glucose solution; and
(g) repeating the steps (b)-(f) after the step (f).
2. The method for continuously detecting glucose concentration in a sample as claimed in claim 1 , wherein the polysaccharide is covered on the surface of the transducer through a process of physical adsorption, or self assembling, or coating, or covalent binding.
3. The method for continuously detecting glucose concentration in a sample as claimed in claim 1 , wherein in the step (c), carbohydrate binding proteins with receptors that are partially occupied by glucose or carbohydrate binding proteins with receptors that are not occupied by glucose in the mixture binds to the polysaccharide generating a physical response or a chemical response.
4. The method for continuously detecting glucose concentration in a sample as claimed in claim 3 , wherein the physical response or chemical response is determined by the transducer to detect the amount of carbohydrate binding proteins bound to the polysaccharide.
5. The method for continuously detecting glucose concentration in a sample as claimed in claim 3 , wherein the physical response or chemical response is a mass change, a viscosity change or a density change.
6. The method for continuously detecting glucose concentration in a sample as claimed in claim 3 , wherein the carbohydrate binding protein comprises a conjugate used as a signal source of the physical response or chemical response, or a sensitizer for enhancing the physical response or chemical response.
7. The method for continuously detecting glucose concentration in a sample as claimed in claim 1 , wherein the transducer comprises a mass sensitive element, an optical element, an electrical element, an electrochemical element or an acoustic element.
8. The method for continuously detecting glucose concentration in a sample as claimed in claim 1 , wherein the transducer comprises a quartz crystal microbalance (QCM) or a surface plasmon resonance transducer (SPR transducer) or a surface acoustic wave element (SAW element).
9. The method for continuously detecting glucose concentration in a sample as claimed in claim 1 , wherein the polysaccharide comprises a dextran or a dextrin.
10. The method for continuously detecting glucose concentration in a sample as claimed in claim 1 , wherein the carbohydrate binding protein comprises a lectin.
11. The method for continuously detecting glucose concentration in a sample as claimed in claim 1 , wherein the carbohydrate binding protein comprises a concanavaline A (Con A), a wheat germ agglutintin (WGA), a peanut agglutinin (PNA) or a soybean agglutinin (SBA).
12. The method for continuously detecting glucose concentration in a sample as claimed in claim 1 , wherein a concentration of the high concentration glucose solution is about 400-2000 mg/dl.
13. A kit for continuously detecting glucose concentration in a sample, comprising:
a biosensor comprising a transducer and a polysaccharide covered on a surface of the transducer;
a reactive solution for reacting with the sample, wherein the reactive solution comprises a carbohydrate binding protein solution with a particular concentration; and
a washing solution for refreshing the surface of the transducer, wherein the washing solution comprises a high concentration glucose solution.
14. The kit for continuously detecting glucose concentration in a sample as claimed in claim 13 , wherein the transducer comprises a mass sensitive element, an optical element, an electrical element, an electrochemical element or an acoustic element.
15. The kit for continuously detecting glucose concentration in a sample as claimed in claim 13 , wherein the transducer comprises a quartz crystal microbalance (QCM) or a surface plasmon resonance transducer (SPR transducer) or a surface acoustic wave element (SAW element).
16. The kit for continuously detecting glucose concentration in a sample as claimed in claim 13 , wherein the polysaccharide comprises a dextran or a dextrin.
17. The kit for continuously detecting glucose concentration in a sample as claimed in claim 13 , wherein the carbohydrate binding protein comprises a lectin.
18. The kit for continuously detecting glucose concentration in a sample as claimed in claim 13 , wherein the carbohydrate binding protein comprises a concanavaline A (Con A), a wheat germ agglutintin (WGA), a peanut agglutinin (PNA) or a soybean agglutinin (SBA).
19. The kit for continuously detecting glucose concentration in a sample as claimed in claim 13 , wherein a concentration of the high concentration glucose solution is about 400-2000 mg/dl.
20. A method for using a biosensor, comprising:
(a) providing a biosensor comprising a transducer and a polysaccharide covered on a surface of the transducer;
(b) contacting a mixture of a reactive solution comprising a carbohydrate binding protein solution with a particular concentration and a sample with the biosensor, wherein the carbohydrate binding protein is capable of binding to the polysaccharide and glucose;
(c) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor to obtain glucose concentration of the sample; and
(d) refreshing the surface of the transducer with a washing solution, wherein the washing solution comprises a high concentration glucose solution.
21. The method for using a biosensor as claimed in claim 20 , wherein the polysaccharide is covered on the surface of the transducer through a process of physical adsorption, or self assembling, or coating, or covalent binding.
22. The method for using a biosensor as claimed in claim 20 , further (e) repeating the steps (b)-(d) after the step (d).
23. The method for using a biosensor as claimed in claim 20 , wherein in the step (b), a carbohydrate binding protein with receptors that are partially occupied by glucose or a carbohydrate binding protein with receptors that are not occupied by glucose in the mixture binds to the polysaccharide generating a physical response or chemical response.
24. The method for using a biosensor as claimed in claim 23 , wherein the physical response or chemical response is determined by the transducer to detect the amount of carbohydrate binding proteins bound to the polysaccharide.
25. The method for using a biosensor as claimed in claim 23 , wherein the carbohydrate binding protein comprises a conjugate used as a source of the physical response or chemical response, or a sensitizer for enhancing the physical response or chemical response.
26. The method for using a biosensor as claimed in claim 20 , wherein the transducer comprises a mass sensitive element, an optical element, an electrical element, an electrochemical element or an acoustic element.
27. The method for using a biosensor as claimed in claim 20 , wherein the transducer comprises a quartz crystal microbalance (QCM) or a surface plasmon resonance transducer (SPR transducer) or a surface acoustic wave element (SAW element).
28. The method for using a biosensor as claimed in claim 20 , wherein the polysaccharide comprises a dextran or a dextrin.
29. The method for using a biosensor as claimed in claim 20 , wherein the carbohydrate binding protein comprises a lectin.
30. The method for using a biosensor as claimed in claim 20 , wherein the carbohydrate binding protein comprises a concanavaline A (Con A), a wheat germ agglutintin (WGA), a peanut agglutinin (PNA) or a soybean agglutinin (SBA).
31. The method for using a biosensor as claimed in claim 20 , wherein a concentration of the high concentration glucose solution is about 400-2000 mg/dl.