Circovirus sequences associated with piglet weight loss disease (PWD)
View Patent ↗The genome sequences and the nucleotide sequences coding for the PWD circovirus polypeptides, such as the circovirus structural and non-structural polypeptides, vectors including the sequences, and cells and animals transformed by the vectors are provided. Methods for detecting the nucleic acids or polypeptides, and kits for diagnosing infection by a PWD circovirus, also are provided. Method for selecting compounds capable of modulating the viral infection are further provided. Pharmaceutical, including vaccine, compositions for preventing and/or treating viral infections caused by PWD circovirus and the use of vectors for preventing and/or treating diseases also are provided.
1. An isolated polypeptide expressed by a baculovirus containing and expressing an exogenous nucleotide sequence, wherein the nucleotide sequence encodes an immunogenic polypeptide whose nucleotide sequence has at least 90% identity to the nucleotide sequence encoding ORF′2 (SEQ ID NO:25).
2. The isolated polypeptide of claim 1 , wherein the nucleotide sequence that encodes the immunogenic polypeptide has at least 95% identity to the nucleotide sequence encoding ORF′2 (SEQ ID NO:25).
3. The isolated polypeptide of claim 1 , wherein the nucleotide sequence that encodes the immunogenic polypeptide has at least 98% identity to the nucleotide sequence encoding ORF′2 (SEQ ID NO:25).
4. An isolated polypeptide expressed by a baculovirus containing and expressing an exogenous nucleotide sequence, wherein the nucleotide sequence encodes an immunogenic polypeptide comprising at least 5 contiguous amino acids of ORF′2 (SEQ ID NO:26).
5. The isolated polypeptide of claim 4 , wherein the immunogenic polypeptide comprises at least 10 contiguous amino acids of ORF′2 (SEQ ID NO:26).
6. The isolated polypeptide of claim 4 , wherein the immunogenic polypeptide comprises at least 15 contiguous amino acids of ORF′2 (SEQ ID NO:26).
7. The isolated polypeptide of claim 4 , wherein the immunogenic polypeptide comprises at least 25 contiguous amino acids of ORF′2 (SEQ ID NO:26).
8. An isolated polypeptide expressed by a baculovirus containing and expressing an exogenous nucleotide sequence, wherein the nucleotide sequence encodes an immunogenic polypeptide having at least 90% identity to ORF′2 (SEQ ID NO:26).
9. The isolated polypeptide of claim 8 , wherein the immunogenic polypeptide has at least 95% identity to ORF′2 (SEQ ID NO:26).
10. The isolated polypeptide of claim 8 , wherein the immunogenic polypeptide has at least 98% identity to ORF′2 (SEQ ID NO:26).
11. A vaccine comprising, in an acceptable vehicle, a polypeptide according to any one of claims 1 - 10 .