LIVER STEM CELLS: ISOLATION OF HEPATIC PROGENITOR CELLS FROM THE HUMAN GALL BLADDER
Methods of preparing multipotent cell populations from gallbladder are provided. Also provided are enriched multipotent cell populations from gallbladder and methods of differentiating the multipotent cell populations to prepare, for example, hepatocytes.
1 . A progenitor cell preparation comprising gallbladder cells enriched for EpCAM+ cells.
2 . The enriched progenitor cell population of claim 1 further enriched by expanding the EpCAM+ cells in culture.
3 . The enriched progenitor cell population of claim 1 comprising EpCam+, CD133+, CD49f+, CD13− cells.
4 . The enriched progenitor cell population of claim 1 further enriched for CD133+, CD49f+, CD13− cells.
5 . A method of preparing an enriched progenitor cell population, comprising:
a. dissociating gallbladder cells; and
b. enriching the gallbladder cells for EpCAM+ cells.
6 . The method of claim 5 , further comprising expanding the EpCAM+ cells in culture.
7 . The method of claim 6 in which the cells are expanded on a feeder layer.
8 . The method of claim 7 in which the feeder layer comprises stromal cells (e.g., fibroblasts).
9 . The method of claim 7 in which the feeder layer comprises epithelial cells.
10 . The method of claim 9 in which the epithelial cells are gall bladder epithelial cells.
11 . The method of claim 10 in which the gall bladder epithelial cells are an immortalized cell line.
12 . The method of claim 7 in which the feeder layer is irradiated to prevent growth of the feeder layer.
13 . The method of claim 12 in which the feeder layer is γ-irradiated.
14 . The method of claim 5 , in which the gallbladder cells are enriched by binding the cells with an anti-EpCAM binding reagent and physically separating the cells that are bound to the anti-EpCAM binding reagent.
15 . The method of claim 14 in which the anti-EpCAM binding reagents are attached to a substrate and the method comprises contacting the dissociated gallbladder cells are contacted with the substrate-bound binding reagents, washing unbound cells from the substrate; and eluting bound cells from the substrate.
16 . The method of claim 14 in which the anti-EpCAM binding reagents are fluorescently labeled and the method comprises sorting the cells in a fluorescence activated cell sorter.
17 . The method of claim 14 in which the anti-EpCam binding reagents are an antibody or a fragment thereof.
18 . The method of claim 5 further comprising enriching the gallbladder cells for CD133+, CD49f+, CD13− cells.
19 . A stem cell line prepared according to the method of claim 5 .
20 . A method of making a hepatocyte, comprising growing EpCAM+ progenitor cells enriched from human gall bladder cells in the presence of one or more of hepatocyte growth factor (HGF), epidermal growth factor (EGF), dexamethasone and transforming growth factor.