Terminus-Specific DNA Modification Using Random-Sequence Template Oligonucleotides
A method is provided for analyzing DNA molecules having unknown 3′ terminal sequences. The method involves contacting a DNA molecule with a plurality of template oligonucleotides blocked at their 3′ termini such that the template oligonucleotides are not extendable by DNA polymerase. The 3′ proximal portions of each of the template oligonucleotides comprise a region of random sequence and the 5′ proximal portions of each of the template oligonucleotides comprise the complement of a tag sequence. The DNA molecule and the template oligonucleotides are combined under conditions wherein the 3′ terminus of the DNA molecule hybridizes to the 3′ proximal portion of a template oligonucleotide and is extended by a DNA polymerase to produce a DNA molecule comprising a 3′ terminal tag sequence, and wherein the template oligonucleotide is not extended.
1 - 20 . (canceled)
21 . A method of analyzing a DNA molecule having an unknown 3′ terminal sequence, comprising:
a) providing a sample comprising at least one DNA molecule having a 3′ terminus of unknown sequence;
b) combining said sample with a mixture comprising a plurality of template oligonucleotides blocked at their 3′ termini such that said plurality of template oligonucleotides are not extendable by a DNA- or RNA-directed DNA polymerase, wherein 3′ proximal portions of said plurality of template oligonucleotides each comprise a region of random sequence and wherein 5′ proximal portions of said plurality of template oligonucleotides each comprise the complement of a tag sequence, and under conditions wherein:
i) said 3′ terminus of said DNA molecule hybridizes to the 3′ proximal portion of a template oligonucleotide, and
ii) said 3′ terminus of said DNA molecule is extended by a DNA- or RNA-directed DNA polymerase to produce a DNA molecule comprising a 3′ terminal tag sequence, and
iii) said template oligonucleotide is not extended by said DNA- or RNA-directed DNA polymerase; and
c) detecting said DNA comprising said 3′ terminal tag sequence.
22 . The method of claim 21 , wherein said detecting comprises amplification.
23 . The method of claim 21 , wherein said detecting comprises sequencing.
24 . The method of claim 21 , wherein said detecting comprises hybridizing said nucleic acid comprising said 3′ terminal tag sequence to an oligonucleotide primer, said primer complementary to at least a portion of said 3′ terminal tag sequence.
25 . The method of claim 21 , further comprising a step of separating said DNA molecule comprising a 3′ terminal tag sequence and said template oligonucleotide.
26 . The method of claim 21 , further comprising a step of purifying said DNA molecule comprising a 3′ terminal tag sequence from said plurality of template oligonucleotides.
27 . The method of claim 22 , wherein said amplification comprises PCR.
28 . The method of claim 21 , wherein said polymerase comprises a DNA-directed DNA polymerase.
29 . The method of claim 21 , wherein said polymerase comprises a reverse transcriptase.