NOVEL RECEPTOR THAT BINDS CTLA-8
Isolated receptors for CTLA-8, DNAs encoding such receptors, and pharmaceutical compositions made therefrom, are disclosed. The isolated receptors can be used to inhibit an immune response.
1 . An isolated DNA selected from the group consisting of:
(a) a DNA having a nucleotide sequence encoding an amino acid sequence of amino acids 1 through 322 of SEQ ID NO.: 2; and
(b) DNA molecules capable of hybridization to the clones of (a) under stringent conditions and which encode CTLA-8R capable of binding CTLA-8.
2 . An isolated DNA according to claim 1 which encodes a soluble CTLA-8R.
3 . An isolated DNA selected from the group consisting of:
(a) a DNA having a nucleotide sequence encoding an amino acid sequence of amino acids 1 through 322 of SEQ ID NO.: 2; and
(b) DNA molecules encoding peptides that are at least about 70% identical in amino acid sequence to the amino acid sequence of SEQ ID NO:2.
4 . A recombinant expression vector comprising a DNA sequence according to claim 1 .
5 . A recombinant expression vector comprising a DNA sequence according to claim 2 .
6 . A recombinant expression vector comprising a DNA sequence according to claim 3 .
7 . A host cell transformed or transfected with an expression vector according to claim 4 .
8 . A host cell transformed or transfected with an expression vector according to claim 5 .
9 . A host cell transformed or transfected with an expression vector according to claim 6 .
10 . A process for preparing a CTLA-8R or an analog thereof, comprising culturing a host cell according to claim 7 under conditions promoting expression.
11 . A process for preparing a CTLA-8R or an analog thereof, comprising culturing a host cell according to claim 8 under conditions promoting expression.
12 . A process for preparing a CTLA-8R or an analog thereof, comprising culturing a host cell according to claim 9 under conditions promoting expression.
13 . An isolated and purified biologically active CTLA-8 receptor (CTLA-8R) protein.
14 . An isolated and purified CTLA-8R protein according to claim 13 , consisting essentially of human CTLA-8R.
15 . An isolated and purified CTLA-8R according to claim 13 , consisting essentially of soluble CTLA-8R.
16 . A composition comprising a CTLA-8R protein according to claim 13 , and a suitable diluent or carrier.
17 . A method for regulating an immune or inflammatory response in a mammal, comprising administering an effective amount of a composition according to claim 16 .
18 . An assay method for detection of CTLA-8 and homologs thereof, or CTLA-8R and homologs thereof, or the interaction thereof, comprising use of a protein composition according to claim 16 .
19 . Antibodies immunoreactive with CTLA-8R.