Reagent compositions for use in electrochemical detection
View Patent ↗Biological reagent compositions with improved sensitivity to the concentration of blood glucose in patient samples for use in measuring systems and methods. The reagent compositions comprise a glucose oxidoreductase enzyme, a flavin nucleoside coenzyme and a mediator formulation. The mediator formulation comprises at least one electroactive organic molecule and at least one coordination complex.
1. A reagent for detecting an analyte, comprising:
a glucose oxidoreductase;
a coenzyme selected from a flavin nucleoside and a nicotinamide nucleotide; and
a mediator formulation comprising at least one electroactive organic molecule independently selected from a phenoxazine or a phenoxazinium salt, and at least one coordination complex selected from osmium and ruthenium complexes.
2. The reagent according to claim 1 , wherein the coenzyme is flavin adenine dinucleotide.
3. The reagent according to claim 1 , wherein the glucose oxidoreductase is glucose dehydrogenase.
4. The reagent according to claim 1 , wherein the at least one electroactive organic molecule is independently selected from a benzophenoxazine or a benzophenoxazinium salt.
5. The reagent according to claim 1 , wherein the at least one coordination complex is a ruthenium complex.
6. The reagent according to claim 1 , wherein the coenzyme is nicotinamide adenine dinucleotide.
7. A reagent for detecting an analyte, comprising:
a glucose oxidoreductase;
a coenzyme selected from a flavin nucleoside and a nicotinamide nucleotide; and
a mediator formulation comprising at least one electroactive organic molecule independently selected from a benzophenoxazine or a benzophenoxazinium salt, and at least one coordination complex independently selected from osmium and ruthenium complexes.
8. The reagent according to claim 7 , wherein the glucose oxidoreductase is a glucose dehydrogenase.
9. The reagent according to claim 7 , wherein the coenzyme is flavin adenine dinucleotide.
10. The reagent according to claim 7 , wherein the coordination complex is a ruthenium complex.
11. The reagent according to claim 7 , wherein the at least one electroactive organic molecule is independently selected from 9-(dimethylamino)benzophenoxazin-7-ium chloride, N-(9H-benzophenoxazin-9-ylidene)-N-methylmethanaminium chloride, or 8-dimethylamino-2,3-benzophenoxazine.
12. The reagent according to claim 7 , wherein the coenzyme is nicotinamide adenine dinucleotide.
13. A reagent for detecting an analyte, comprising:
a glucose oxidoreductase;
a coenzyme selected from a flavin nucleoside and a nicotinamide nucleotide; and
a mediator formulation comprising at least one electroactive organic molecule independently selected from a benzophenoxazine or a benzophenoxazinium salt, and at least one ruthenium coordination complex.
14. The reagent according to claim 13 , wherein the glucose oxidoreductase is a glucose dehydrogenase.
15. The reagent according to claim 13 , wherein the flavin nucleoside coenzyme is flavin adenine dinucleotide.
16. The reagent according to claim 13 , wherein the coordination complex is ruthenium hexamine.
17. The reagent according to claim 13 , wherein the at least one electroactive organic molecule is independently selected from 9-(dimethylamino)benzophenoxazin-7-ium chloride, N-(9H-benzophenoxazin-9-ylidene)-N-methylmethanaminium chloride, 8-dimelthylamino-2,3-benzophenoxazine hemi (zinc chloride) salt, 7-dimethylamino-1,2-benzophenoxazine or 8-dimethylamino-2,3-benzophenoxazine.
18. The reagent according to claim 13 , wherein the coenzyme is nicotinamide adenine dinucleotide.
19. A reagent for detecting an analyte, comprising:
glucose dehydrogenase;
a coenzyme selected from flavin adenine dinucleotide and nicotinamide adenine dinucleotide; and
a mediator formulation comprising at least one electroactive organic molecule independently selected from a benzophenoxazine or a benzophenoxazinium salt, and at least one ruthenium complex.
20. The reagent according to claim 19 , wherein the at least one electroactive organic molecule is independently selected from 9-(dimethylamino)benzophenoxazin-7-ium chloride, N-(9H-benzophenoxazin-9-ylidene)-N-methylmethanaminium chloride, 8-dimelthylamino-2,3-benzophenoxazine hemi (zinc chloride) salt, 7-dimethylamino-1,2-benzophenoxazine or 8-dimethylamino-2,3-benzophenoxazine.
21. The reagent according to claim 19 , wherein the at least one ruthenium complex is ruthenium hexamine.
22. The reagent according to claim 20 , wherein the at least one ruthenium complex is ruthenium hexamine.