Methods for preserving organs and tissues
View Patent ↗The invention relates to a method for preserving an organ or tissue comprising contacting the organ or tissue with an effective amount of a kallikrein inhibitor and solutions useful for such a method. Also provided is a method for reducing reperfusion injury of an organ during surgery and/or following removal of the organ from a subject comprising placing the organ in an organ storage and preservative solution, wherein the solution comprises a kallikrein inhibitor.
1. A method for reducing kallikrein activity in a removed organ or tissue, the method comprising: contacting the organ or tissue, ex vivo, with an effective amount of a polypeptide comprising amino acids 3-60 of SEQ ID NO: 2.
2. The method of claim 1 , wherein the polypeptide comprises SEQ ID NO: 2.
3. The method of claim 1 , wherein the organ or tissue is selected from the group consisting of heart, lung, kidney, pancreas, liver, intestine, endothelial tissue, vascular tissue and skin.
4. The method of claim 1 , wherein the polypeptide consists of amino acids 3-60 of SEQ ID NO: 2.
5. The method of claim 1 , wherein the polypeptide consists of SEQ ID NO: 2.
6. A method for reducing reperfusion injury in a removed organ or tissue, the method comprising: contacting the organ or tissue, ex vivo, with an effective amount of a polypeptide comprising amino acids 3-60 of SEQ ID NO: 2.
7. The method of claim 6 , wherein the polypeptide comprises SEQ ID NO: 2.
8. The method of claim 6 , wherein the organ or tissue is selected from the group consisting of heart, lung, kidney, pancreas, liver, intestine, endothelial tissue, vascular tissue and skin.
9. The method of claim 6 , wherein the polypeptide consists of amino acids 3-60 of SEQ ID NO: 2.
10. The method of claim 6 , wherein the polypeptide consists of SEQ ID NO: 2.