Kinase and phosphatase assays
Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.
1. A composition of matter capable of being a serine/threonine kinase substrate, comprising:
(a) a peptide having a length from eight to fifty amino acids, the peptide comprising FSVPPS*PD, where S* is a phosphorylated serine (SEQ ID NO:85); and
(b) a first detectable moiety associated with the peptide.
2. The composition of claim 1 , wherein the first detectable moiety is covalently linked to the peptide.
3. The composition of claim 1 , wherein the peptide comprises a protease cleavage site.
4. The composition of claim 3 , wherein the protease cleavage site is selected from the group consisting of a chymotrypsin protease cleavage site, a caspase 3 protease cleavage site, a cathepsin G protease cleavage site, a trypsin protease cleavage site, an elastase protease cleavage site, an endoproteinase Asp-N protease cleavage site, and an endoproteinase Glu-N protease cleavage site.
5. The composition of claim 1 , wherein the peptide has a length selected from the group consisting of from 8 to 25 amino acids and from 8 to 15 amino acids.
6. The composition of claim 1 , further comprising a second detectable moiety associated with the peptide.
7. The composition of claim 1 , wherein the composition exhibits an optical property, a magnetic property, or a radioactive property.
8. The composition of claim 7 , wherein the composition exhibits an optical property selected from the group consisting of a molar extinction coefficient at an excitation wavelength, a quantum efficiency, an excitation spectrum, an emission spectrum, an excitation wavelength maximum, an emission wavelength maximum, a ratio of excitation amplitudes at two wavelengths, a ratio of emission amplitudes at two wavelengths, an excited state lifetime, an anisotropy, a polarization of emitted light, a resonance energy transfer, and a quenching of emission at a wavelength.
9. The composition of claim 7 , wherein the optical property is selected from the group consisting of a fluorescence excitation spectrum, a fluorescence emission spectrum, a fluorescence excitation wavelength maximum, a fluorescence emission wavelength maximum, a ratio of fluorescence excitation amplitudes at two wavelengths, a ratio of fluorescence emission amplitudes at two wavelengths, a fluorescence excited state lifetime, a fluorescence anisotropy, and a quenching of fluorescence emission at a wavelength.
10. The composition of claim 6 , wherein the first or second detectable moieties are selected from the group consisting of 5-FAM, 6-FAM, 7-hydroxycoumarin-3-carboxamide, 6-chloro-7-hydroxycoumarin-3-carboxamide, fluorescein-5-isothiocyanate, dichlorotriazinylaminofluorescein, tetramethylrhodamine-5-isothiocyanate, tetramethylrhodamine-6-isothiocyanate, succinimidyl ester of 5-carboxyfluorescein, succinimidyl ester of 6-carboxyfluorescein, 5-carboxytetramethylrhodamine, 6-carboxymethylrhodamine, fluorescein maleimide, and 7-amino-4-methylcoumarin-3-acetic acid.
11. The composition of claim 6 , wherein the first detectable moiety, the second detectable moiety or the first and second detectable moiety is a member of a specific binding pair.
12. The composition of claim 6 , wherein the first detectable moiety and the second detectable probe moiety form a dark quenching RET pair or a FRET pair.
13. The composition of claim 6 , wherein the first detectable moiety is 7-hydroxycoumarin-3-carboxamide and the second detectable moiety is 5-FAM.
14. The composition of claim 6 , wherein the first or second detectable probe moiety is covalently linked to the peptide via a linker (L).
15. The composition of claim 14 , wherein the L is selected from the group consisting of GABA, diaminopentanyl, and aminohexanoyl.
16. A method for identifying a modulator of activity of a serine/threonine kinase, comprising:
(a) forming a mixture of a serine/threonine protein kinase, a composition of claim 1 , and a test compound;
(b) contacting the mixture with a protease to form a protease mixture; and
(c) comparing a measurable property in the protease mixture to the measurable property in a control mixture, wherein the test compound is identified as a modulator of activity of the kinase if the measurable property in the protease mixture is different from the measurable property in the control mixture.
17. A method for identifying a modulator of activity of a serine/threonine phosphatase, comprising:
(a) contacting a mixture of a serine/threonine protein phosphatase, a composition of claim 1 , and a test compound to form a phosphatase mixture, wherein the composition of claim 1 comprises a phosphorylated threonine or serine amino acid;
(b) contacting the phosphatase mixture with a protease to form a protease mixture; and
(c) comparing a measurable property in the protease mixture to the measurable property in a control protease mixture, wherein the test compound is identified as a modulator of activity of the phosphatase if the measurable property in the protease mixture is different from the measurable property in the control mixture.
18. An article of manufacture comprising:
(a) packaging matter; and
(b) a composition of matter according to claim 1 associated with the packaging material.
19. The article of claim 18 , further comprising at least one member selected form the group consisting of a serine/threonine protein kinase, a serine/threonine protein phosphatase, a protease and ATP.
20. The composition of claim 1 , wherein the peptide comprises PRPFSVPPS*PDK (SEQ ID NO: 86).