DIG-3 insecticidal Cry toxins
View Patent ↗DIG-3 Cry toxins, polynucleotides encoding such toxins, and transgenic plants that produce such toxins are useful to control insect pests.
1. An isolated polypeptide comprising a core toxin segment comprising the amino acid sequence of residues 113 to 643 of SEQ ID NO: 2, wherein said polypeptide has insecticidal activity.
2. An isolated polypeptide comprising a core toxin segment selected from the group consisting of (a) a polypeptide comprising the amino acid sequence of residues 73 to 643 of SEQ ID NO: 2; and (b) a polypeptide comprising an amino acid sequence having at least 97% sequence identity to the amino acid sequence of residues 73 to 643 of SEQ ID NO:2; wherein said polypeptide has insecticidal activity.
3. The isolated polypeptide of claim 2 comprising a core toxin segment selected from the group consisting of (a) a polypeptide comprising the amino acid sequence of residues 1 to 643 of SEQ ID NO: 2; and (b) a polypeptide comprising an amino acid sequence having at least 97% sequence identity to the amino acid sequence of residues 1 to 643 of SEQ ID NO:2; wherein said polypeptide has insecticidal activity.
4. A plant comprising the polypeptide of claim 2 .
5. A method for controlling a pest population comprising contacting said population with a pesticidally effective amount of the polypeptide of claim 2 .
6. An isolated polynucleotide that encodes the polypeptide of claim 2 .
7. The isolated polynucleotide of claim 6 wherein said polynucleotide comprises SEQ ID NO: 1 or SEQ ID NO:3.
8. The polypeptide of claim 2 , wherein said polypeptide comprises SEQ ID NO: 2 or SEQ ID NO: 5.
9. A DNA construct comprising the polynucleotide of claim 2 operably linked to a promoter that is not derived from Bacillus thuringiensis and is capable of driving expression in a plant.
10. A transgenic plant that comprises the DNA construct of claim 9 stably incorporated into its genome.
11. A method for protecting a plant from a pest comprising introducing into said plant the construct of claim 9 .
12. The polypeptide of claim 2 wherein said polypeptide has insecticidal activity against European corn borer.