Transient expression of immunomodulatory polypeptides for the prevention and treatment of autoimmune disease, allergy and transplant rejection
A method is provided for treating or preventing an undesired immune response in a patient, comprising: administering to said patient, cells that transiently express, and/or that are transfected with mRNA encoding, one or more polypeptides selected from the group consisting of an IL-4 receptor agonist, an IFN-γ receptor antagonist, an IFN-α receptor antagonist, an IL-12 receptor antagonist, an IL-23 receptor antagonist, and a TNF antagonist. Preferably, the cells selectively accumulate in one or more secondary lymphoid tissues at or proximate to the site of the undesired immune response. Related compositions are provided. The methods and compositions are useful for the treatment or prevention of undesired immune responses including, but not limited to, transplant rejection, autoimmune disease, allergy and immune responses directed against therapeutic compositions.
1. A method for treating an undesired immune response in a patient, comprising: administering to said patient, cells transfected with mRNA encoding an IL-4 receptor agonist, wherein the undesired immune response is an autoimmune disease, wherein said autoimmune disease is insulin dependent diabetes mellitus.
2. The method of claim 1 , wherein said mRNA encodes IL-4.
3. The method of claim 1 , wherein said cells are co-transfected with mRNA encoding a homing polypeptide.
4. The method of claim 1 , wherein said cells selectively accumulate in one or more secondary lymphoid tissues at or proximate to the site of the undesired immune response.
5. The method of claim 1 , wherein the cells are dendritic cells.
6. A method for treating an undesired immune response in a patient, comprising: administering to said patient, cells transfected with mRNA encoding an IL-4 receptor agonist, wherein the undesired immune response is the presence of an anti-islet cell antibody in said patient.
7. The method of claim 6 , wherein said anti-islet cell antibody is specific for glutamate decarboxylase (GAD), insulinoma associated peptide-2 (IA-2) or insulin.
8. The method of claim 1 , wherein said administration is intravenous, intraperitoneal, subcutaneous, intradermal, intramuscular or intranodal.
9. The method of claim 1 , wherein the cell expresses a heterologous homing polypeptide.