Methods and compositions for inhibition of BCL6 repression
View Patent ↗The invention is directed to a compound that binds to a BCL6 lateral groove and prevents binding of a corepressor to the lateral groove. The present invention is further directed to methods for blocking corepressor binding to a BCL6 lateral groove, methods for inhibiting BCL6 repression in a mammalian cell, and methods for treating a mammal with cancer, wherein the cancer requires BCL6 repression. The present invention is further directed to polypeptides comprising a portion of the corepressor binding site for BCL6 and related polynucleotides and vectors.
1. A compound that binds to a BCL6 lateral groove and prevents binding of a corepressor to the lateral groove, wherein the compound comprises a peptide or mimetic consisting of the amino acid sequence GRGIEHISR (SEQ ID NO:1) or the amino acid sequence GRGIEHISRG (SEQ ID NO:2).
2. The compound of claim 1 , wherein the peptide or mimetic consists of the amino acid sequence GRGIEHISR (SEQ ID NO:1).
3. The compound of claim 1 , wherein the peptide or mimetic consists of the amino acid sequence GRGIEHISRG (SEQ ID NO:2).
4. The compound of claim 1 , wherein the compound further comprises a TAT sequence and a fusogenic sequence having the amino acid sequence NH 2 -GLFGAIAGFIENGWEGMIDG-OH (SEQ ID NO:8).
5. The compound of claim 4 , wherein the peptide or mimetic is directly linked to the TAT sequence, and the TAT sequence is directly linked to the fusogenic sequence.
6. The compound of claim 4 , wherein the TAT sequence is directly linked to the peptide or mimetic, and the peptide or mimetic is directly linked to the fusogenic sequence.
7. The compound of claim 4 , wherein the TAT sequence is directly linked to the fusogenic sequence, and the fusogenic sequence is directly linked to the peptide or mimetic.
8. The compound of claim 1 in a pharmaceutically acceptable excipient.
9. A method for blocking corepressor binding to a BCL6 lateral groove, the method comprising contacting the BCL6 with the compound of claim 1 , wherein the BCL6 is in a lymphoma cell or a breast cancer cell.
10. The method of claim 9 , wherein the lymphoma cell or breast cancer cell is in a human.
11. The method of claim 9 , wherein the cell is a lymphoma cell.
12. The method of claim 9 , wherein the cell is a breast cancer cell.
13. A method of inhibiting BCL6 repression in a mammalian cell, method comprising treating the cell with the compound of claim 1 , wherein the cell is a lymphoma cell or a breast cancer cell.
14. The method of claim 13 , wherein the cell is a lymphoma cell.
15. The method of claim 13 , wherein the cell is a breast cancer cell.
16. The method of claim 13 , wherein the cell is in a mammal.
17. The method of claim 16 , wherein the mammal is a human.
18. A method for treating a mammal with cancer, wherein the cancer requires BCL6 repression, the method comprising administering the compound of claim 8 to the mammal, wherein the cancer is a lymphoma or a breast cancer.
19. The method of claim 18 , wherein the mammal is a human.
20. The method of claim 18 , wherein the cancer is a lymphoma.
21. The method of claim 18 , wherein the cancer is a breast cancer.
22. An isolated polypeptide comprising the amino acid sequence GRGIEHISR (SEQ ID NO:1).