Chromatography inspection apparatus and method for judging degradation of chromatography specimen
View Patent ↗By measuring a luminance difference between predetermined two points or a luminance variation in a predetermined region in a state in which a liquid sample is developed in a chromatography specimen 1 , and comparing the luminance difference or the luminance variation with a preset reference value, it is possible to automatically detect degradation such as a decrease in hydrophilicity in the lower portion of a liquid-impermeable sheet material 8 during a chromatography inspection, thereby enabling an accurate inspection.
1. A chromatography inspection apparatus for conducting an inspection by developing a liquid sample in a developing layer of a chromatography specimen on which is a liquid-impermeable sheet material is bonded, melting a labeling reagent retained in a labeling reagent retaining part, and coloring a reagent fixing part by the labeling reagent,
the chromatography inspection apparatus comprising:
an image pickup device for imaging the chromatography specimen in a state in which the liquid sample has developed; and
a measuring section for judging whether the chromatography specimen has been degraded or not based on an imaging result,
wherein when the measuring section judges that the chromatography specimen has been degraded, a warning is issued, and
wherein the measuring section determines, based on the imaging result, a difference in luminance between the labeling reagent retaining part and a region located downstream of the labeling reagent retaining part in a developing direction, and the measuring section judges that the chromatography specimen has been degraded when the difference is larger than a preset reference value.
2. The chromatography inspection apparatus according to claim 1 , wherein when the measuring section judges that the chromatography specimen has been degraded, an inspection operation is stopped.
3. A chromatography inspection apparatus for conducting an inspection by developing a liquid sample in a developing layer of a chromatography specimen on which a liquid-impermeable sheet material is bonded, melting a labeling reagent retained in a labeling reagent retaining part, and coloring a reagent fixing part by the labeling reagent,
the chromatography inspection apparatus comprising:
an image pickup device for imaging the chromatography specimen in a state in which the liquid sample has developed; and
a measuring section for judging whether the chromatography specimen has been degraded or not based on an imaging result,
wherein when the measuring section judges that the chromatography specimen has been degraded, a warning is issued, and
wherein the measuring section determines, based on the imaging result, a difference in luminance between a region where the liquid sample has developed and a region located in a developing direction downstream of an end of the region where the liquid sample has developed, and the measuring section judges that the chromatography specimen has been degraded when the difference is smaller than a preset reference value.
4. The chromatography inspection apparatus according to claim 3 , wherein when the measuring section judges that the chromatography specimen has been degraded, an inspection operation is stopped.
5. A chromatography inspection apparatus of conducting an inspection by developing a liquid sample in a developing layer of a chromatography specimen on which a liquid-impermeable sheet material is bonded, melting a labeling reagent retained in a labeling reagent retaining part, and coloring a reagent fixing part by the labeling reagent,
the chromatography inspection apparatus comprising:
an image pickup device for imaging the chromatography specimen in a state in which the liquid sample has developed; and
a measuring section for judging, based on an imaging result, whether the chromatography specimen has been degraded by a decrease in hydrophilicity of a portion of the developing layer of the chromatography specimen contacting the liquid-impermeable sheet material or not,
wherein when the measuring section judges that the chromatography specimen has been degraded, a warning is issued, and
wherein the measuring section determines, based on the imaging result, a difference in luminance between a region where the liquid-impermeable sheet material is bonded and a region located in a developing direction downstream of a downstream end of the region where the liquid-impermeable sheet material is bonded, and the measuring section judges that the chromatography specimen has been degraded by the decrease in hydrophilicity of the developing layer of the chromatography specimen when the difference is larger than a preset reference value.
6. A method of judging degradation of a chromatography specimen, in which degradation of the chromatography specimen is detected concurrently with a chromatography inspection conducted by developing a liquid sample in a developing layer of the chromatography specimen on which a liquid-impermeable sheet material is bonded, melting a labeling reagent retained in a labeling reagent retaining part, and coloring a reagent fixing part by the labeling agent,
the method comprising:
imaging the chromatography specimen in a state in which the liquid sample has developed; and
judging whether the chromatography specimen has been degraded or not based on an imaging result,
wherein when it is judged that the chromatography specimen has been degraded during the inspection, an inspection operation is stopped, and
wherein the judging step comprises:
determining a difference in luminance between the labeling reagent retaining part and a region located downstream of the labeling reagent retaining part in a developing direction after the liquid sample has developed; and
comparing the determined luminance difference with a preset reference value and judging that the chromatography specimen has been degraded when the luminance difference is larger than the reference value.
7. A method of judging degradation of a chromatography specimen, in which degradation of the chromatography specimen is detected concurrently with a chromatography inspection conducted by developing a liquid sample in a developing layer of the chromatography specimen on which a liquid-impermeable sheet material is bonded, melting a labeling reagent retained in a labeling reagent retaining part, and coloring a reagent fixing part by the labeling agent,
the method comprising:
imaging the chromatography specimen in a state in which the liquid sample has developed; and
judging whether the chromatography specimen has been degraded or not based on an imaging result,
wherein when it is judged that the chromatography specimen has been degraded during the inspection, an inspection operation is stopped, and
wherein the judging step comprises:
determining, after the liquid sample has developed, a difference in luminance between a region where the liquid sample has developed and a region located in a developing direction downstream of an end of the region where the liquid sample has developed; and
comparing the determined luminance difference with a preset reference value and judging that the chromatography specimen has been degraded when the luminance difference is smaller than the reference value.
8. A method of judging degradation of a chromatography specimen, in which degradation of the chromatography specimen is detected concurrently with a chromatography inspection conducted by developing a liquid sample in a developing layer of the chromatography specimen on which a liquid-impermeable sheet material is bonded, melting a labeling reagent retained in a labeling reagent retaining part, and coloring a reagent fixing part by the labeling agent,
the method comprising:
imaging the chromatography specimen in a state in which the liquid sample has developed; and
judging, based on an imaging result, whether the chromatography specimen has been degraded by a decrease in hydrophilicity of a portion of the developing layer of the chromatography specimen contacting the liquid-impermeable sheet material or not,
wherein when it is judged that the chromatography specimen has been degraded during the inspection, an inspection operation is stopped, and
wherein the judging step comprises:
determining, after the liquid sample has developed, a difference in luminance between a region where the liquid-impermeable sheet material is bonded and a region located in a developing direction downstream of a downstream end of the region where the liquid-impermeable sheet material is bonded; and
comparing the determined luminance difference with a preset reference value and judging that the chromatography specimen has been degraded by the decrease in hydrophilicity of the developing layer of the chromatography specimen when the luminance difference is larger than the reference value.