Methods for selective targeting
View Patent ↗A selective targeting method is disclosed which comprises contacting a peptide library with an anti-target to allow the peptides to bind; separating non-binding peptides from the anti-target bound peptides, contacting the non-binding anti-target peptides with a target allowing the unbound peptides to bind with the target to form a target-bound peptide complex; separating the target-bound peptide complex from peptides which do not bind to the target, and identifying the target-bound peptides. In one embodiment the target is skin or hair. In another embodiment the anti-target is hair when the target is skin, and the anti-target is skin when the target is hair.
1. A method for screening a peptide library comprising the steps of,
(a) contacting said peptide library with an anti-target under conditions that allow binding between the peptides of said library and said anti-target;
(b) separating anti-target unbound peptides from anti-target bound peptides;
(c) contacting said anti-target unbound peptides with a target under conditions favorable for binding between said anti-target unbound peptides and said target to form a target-bound peptide complex;
(d) separating said target-bound peptide complex from anti-target unbound peptides which do not bind to said target; and
(e) identifying the target-bound peptides on the target-bound peptide complex,
wherein said anti-target is selected from the group consisting of skin tissue and hair, wherein when and said target is skin tissue said anti-target is hair and when said target is hair said anti-target is skin tissue.
2. The method according to claim 1 , wherein steps (a), (b), (c) and (d) are repeated between 2 to 10 times using said target-bound peptides.
3. The method according to claim 1 , wherein the target-bound peptide is not released from the target during the identifying step.
4. The method according to claim 1 , wherein said peptide library is a phage display library.
5. The method according to claim 1 , wherein separating said target-bound peptide further includes an acid elution step.
6. The method according to claim 1 , wherein step (a) and (b) are repeated between 2 to 10 times prior to said contacting step (c).
7. The method according to claim 1 , wherein said identified target-bound peptides are less than 25 amino acids in length.
8. The method according to claim 1 , wherein the identified target-bound peptides have a selectivity of binding to the target compared to binding to the anti-target of at least 20:1.
9. The method according to claim 4 , wherein said identifying step comprises amplifying a nucleic acid coding for said target-bound peptide in a polymerase chain reaction.