Tube for DNA reactions
A reaction tube for use in performing the Polymerase Chain Reaction (PCR) in a thermal cycler, is provided herein such reaction tube includes a hollow tube of heat conducting synthetic plastics material having an open top and a flat bottom formed of an optically-clear material. Such reaction tube also includes a solid plug cap nesting within said hollow tube and to provide a minimized void volume within the hollow tube and to minimize evaporation and condensation of the biological fluid.
1. A reaction tube comprising:
a hollow tube of heat conducting synthetic plastic material comprising:
a) an open top;
b) an upper cylindrical portion;
c) a lower frustoconical portion; and
d) a flat bottom formed of an optically-clear material; and
a solid plug cap, wherein the solid plug cap sealingly nests within the upper cylindrical portion and partially within the lower frustoconical portion of the hollow tube.
2. The reaction tube of claim 1 , wherein the hollow tube further comprises an upper lip on the upper cylindrical portion of the hollow tube.
3. The reaction tube of claim 2 , wherein the upper lip is oval in plane view.
4. The reaction tube of claim 2 , wherein the solid plug cap comprises an upper cylindrical plug region and a lower cylindrical plug region.
5. The reaction tube of claim 4 , wherein the solid plug cap further comprises a middle cylindrical plug region located between the upper and lower cylindrical plug regions.
6. The reaction tube of claim 5 , wherein the upper cylindrical plug region is of a greater diameter than the middle cylindrical plug region.
7. The reaction tube of claim 6 , wherein the middle cylindrical plug region nests within the upper cylindrical portion of the hollow tube and the lower cylindrical plug region nests within the lower frustoconical portion of the hollow tube.
8. The reaction tube of claim 7 , wherein the upper cylindrical plug region rests upon the upper lip of the hollow tube.
9. The reaction tube of claim 6 , wherein the hollow tube has a diameter smaller than the diameter of the upper cylindrical plug region.
10. The reaction tube of claim 5 , wherein the upper cylindrical plug region is of a greater diameter than the middle cylindrical plug region and the middle cylindrical plug region is of a greater diameter than the lower cylindrical plug region.
11. The reaction tube of claim 10 , wherein the middle cylindrical plug region nests within the upper cylindrical portion of the hollow tube and the lower cylindrical plug region nests within the lower frustoconical portion of the hollow tube.
12. The reaction tube of claim 1 , wherein the lower frustroconical portion of the hollow tube has a draft angle of about 5 degrees.