Polyelectrolyte-Coated Size-Exclusion Ion-Exchange Particles
A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.
1 . A solution comprising:
a particle, the particle comprising:
a core comprising ion-exchange material; and
a coating comprising polyelectrolyte material,
wherein the core and coating are adapted to separate DNA sequencing reaction products;
a non-ionic surfactant; and
a stabilizing additive.
2 . The solution of claim 1 , wherein the non-ionic surfactant is adapted to facilitate removal of unincorporated dye terminators from the DNA sequencing reaction.
3 . The solution of claim 2 , wherein the non-ionic surfactant is effective without heating.
4 . The solution of claim 1 , wherein the ion-exchange material is cationic.
5 . The solution of claim 4 , further comprising an anion-exchange particle.
6 . The solution of claim 1 , wherein the ion-exchange material is anionic.
7 . The solution of claim 6 , further comprising a cation-exchange particle.
8 . The solution of claim 1 , wherein the non-ionic surfactant is a zwitter-ionic surfactant.
9 . The solution of claim 8 , wherein the zwitter-ionic surfactant is CHAPS.
10 . The solution of claim 1 , wherein the stabilizing additive is a zwitter-ionic compound.
11 . The solution of claim 10 , wherein the zwitter-ionic compound is betaine.
12 . A method for DNA sequencing, the method comprising:
providing a plurality of particles, wherein each particle comprises a core comprising ion-exchange material and a coating comprising polyelectrolyte material;
contacting the plurality of particles with DNA sequencing reaction products;
providing a non-ionic detergent; and
stabilizing the solution with a zwitter-ionic compound.
13 . The method of claim 12 , wherein the non-ionic detergent is CHAPS.
14 . The method of claim 12 , wherein zwitter-ionic compound is betaine.
15 . The method of claim 12 , further comprising isolating the particles.
16 . The method of claim 15 , further comprising separating the DNA sequencing reaction products remaining in solution with capillary electrophoresis.
17 . The method of claim 12 , further comprising storing the particles, the DNA sequencing reaction products, the non-ionic detergent, and the zwitter-ionic compound for at least eight hours.
18 . A kit for purification of DNA sequencing products, the kit comprising:
a mixture of cationic and anionic particles, wherein at least one of the cationic and anionic type of particles comprise:
a core comprising ion-exchange material; and
a coating comprising polyelectrolyte material,
wherein the core and coating are adapted to separate DNA sequencing reaction products;
a non-ionic surfactant; and
a stabilizing additive.
19 . The kit of claim 18 , wherein the mixture, the surfactant, and the additive are packaged together.
20 . The kit of claim 18 , wherein the mixture, the surfactant, and the additive are packaged separately.
21 . A method for stabilizing DNA sequencing reaction products, the method comprising:
providing DNA sequencing reaction products;
purifying the DNA sequencing reaction products with purification media;
contacting the DNA sequencing reaction products with DI water;
providing a zwitter-ionic stabilizer; and
electrokinetically injecting the DNA sequencing reaction products into a CE system.
22 . The method of claim 21 , further comprising separating the DNA sequencing reaction products from the purification media
23 . The method of claim 21 , wherein the purification media comprises a particle, the particle comprising:
a core comprising ion-exchange material; and
a coating comprising polyelectrolyte material.
24 . The method of claim 21 , wherein the zwitter-ionic stabilizer is betaine.