Genetic loci associated with
The invention relates to methods and compositions for identifying and selecting maize plants with enhanced resistance to Fusarium ear mold. Maize plants generated by the methods of the invention are also a feature of the invention.
1. A method of selecting a maize plant with enhanced Fusarium ear mold resistance comprising:
a. obtaining DNA accessible for analysis;
b. detecting in the maize plant at least one marker allele selected from the group consisting of:
i. a “T” at position 389 of SEQ ID NO:43,
ii. a “C” at position 373 of SEQ ID NO:42,
iii. a “T” at position 141 of SEQ ID NO:44,
iv. a “C” at position 326 of SEQ ID NO:47,
v. a “C” at position 464 of SEQ ID NO:42,
vi. a “C” at position 459 of SEQ ID NO:45,
vii. a “T” at position 113 of SEQ ID NO:46,
viii. a “C” at position 254 of SEQ ID NO:41,
ix. an “A” at position 246 of SEQ ID NO: 173,
x. an “A” at position 408 of SEQ ID NO:174,
xi. an “A” at position 524 of SEQ ID NO:174, and
xii. a “G” at position 538 of SEQ ID NO:175; and
c. selecting said maize plant that has the at least one marker allele.
2. A method of identifying a maize plant that displays enhanced resistance to Fusarium ear mold, the method comprising:
a. detecting in the germplasm of the maize plant a haplotype associated with enhanced resistance to Fusarium ear mold, wherein said haplotype comprises:
i. a “C” at position 254 of SEQ ID NO:41; a “T” at position 389 of SEQ ID NO:43; and a “T” at position 141 of SEQ ID NO:44;
ii. an “A” at position 246 of SEQ ID NO:173, and an “A” at position 408 of SEQ ID NO:174; or
iii. an “A” at position 246 of SEQ ID NO:173; a “T” at position 389 of SEQ ID NO:43; and an “A” at position 408 of SEQ ID NO:174; and
b. selecting said maize plant that has a haplotype comprising (a)(i), (a)(ii), or (a)(iii).
3. A method of selecting a maize plant that displays enhanced resistance to Fusarium ear mold, the method comprising:
a. obtaining a first maize plant that comprises within its genome:
i. a “C” at position 254 of SEQ ID NO:41; a “T” at position 389 of SEQ ID NO:43; and a “T” at position 141 of SEQ ID NO:44;
ii. an “A” at position 246 of SEQ ID NO:173 and an “A” at position 408 of SEQ ID NO:174; or
iii. an “A” at position 246 of SEQ ID NO:173; a “T” at position 389 of SEQ ID NO:43; and an “A” at position 408 of SEQ ID NO:174;
b. crossing said first maize plant to a second maize plant;
c. evaluating progeny plants for said alleles; and
d. selecting progeny plants that possess said alleles.