Methods and devices for detecting the presence of an analyte in a sample
View Patent ↗Sensor assay methods for detecting the presence of an analyte in a sample are provided. Aspects of the methods include providing a sensor, e.g., a proximity sensor, in contact with an assay composition that includes a sample and a proximity label. Next, a capture probe configured to bind to the proximity label and the analyte is introduced into the assay composition to produce a labeled analyte. Following capture probe introduction, a signal is obtained from the sensor to detect the presence of the labeled analyte in the sample. Also provided are sensor devices, including hand-held devices, and kits that find use in practicing the subject methods.
1. A method of detecting the presence of an analyte in a sample, the method comprising:
contacting a magnetic sensor comprising a surface capture ligand that specifically binds to an analyte with an assay composition comprising a sample and a magnetic proximity label;
introducing a capture probe into the assay composition, wherein the capture probe is configured to bind to the magnetic proximity label and the analyte to produce a labeled analyte; and
obtaining a signal from the magnetic sensor to detect the presence of the labeled analyte in the sample,
wherein the method is a wash-free method.
2. The method according to claim 1 , wherein the method comprises producing the assay composition by sequentially contacting the sensor with the sample and the proximity label.
3. The method according to claim 1 , wherein the method comprises combining the sample and the proximity label to produce the assay composition and then contacting the sensor with the assay composition.
4. The method according to claim 1 , wherein the method comprises detecting the presence of two or more analytes in the sample.
5. The method according to claim 1 , wherein the method comprises detecting the cross-reactivity of an analyte or antibody.
6. The method according to claim 5 , wherein the detecting comprises sequentially introducing different capture probes into the assay composition, wherein each different capture probe is configured to specifically bind to a distinct analyte.
7. The method according to claim 5 , further comprising detecting specific binding of each capture probe to its corresponding analyte.
8. The method according to claim 1 , wherein the proximity sensor is a component of a hand-held device.
9. The method according to claim 1 , wherein the presence of the analyte in the sample is detected qualitatively.
10. The method according to claim 1 , wherein the presence of the analyte in the sample is detected quantitatively.
11. The method according to claim 1 , wherein the proximity label is a colloidal proximity label.
12. The method according to claim 11 , wherein the proximity label comprises a magnetic label and a polymer.
13. The method according to claim 12 , wherein the polymer comprises dextran.
14. The method according to claim 1 , wherein the capture probe is configured to non-covalently bind to the proximity label.
15. The method according to claim 14 , wherein the proximity label is functionalized with a first member of a binding pair and the capture probe is functionalized with a complementary member of the binding pair.