IP Library Granted Patent US 8,383,394
Granted Patent B2
US 8,383,394 · App. 12/760,526 · Granted Feb 26, 2013

System and method for electroporating a sample

Inventors: Richard Jarvis (Austin, TX); Mike Byrom (Austin, TX); Dmitriy Ovcharenko (Austin, TX)
Assignee: Applied Biosystems, LLC
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Quick Facts
Patent No.
US 8,383,394
App. No.
12/760,526
Granted
Feb 26, 2013
Kind
B2
Abstract

A system and method are described for electroporating a sample that utilizes one or more sets of electrodes that are spaced apart in order to hold a surface tension constrained sample between the electrodes. The first electrode is connected to the lower body of the system while the second electrode is connected to the upper body. Both electrodes are connected to a pulse generator. Each electrode has a sample contact surface such that the first electrode and the second electrode may be positioned to hold a surface tension constrained sample between the two sample contact surfaces and the sample may receive a selected electric pulse.

Claims (29)

1. An electroporation device comprising:

a base including a base electrode array; and

a top including a top electrode array,

wherein the base includes base fangs.

2. The device of claim 1 wherein the base electrode array is a 96 electrode array of electrodes.

3. The device of claim 2 wherein each of the electrodes of the 96 electrode array includes a contact surface.

4. The device of claim 1 wherein the top electrode array is a 96 electrode array of electrodes.

5. The device of claim 4 wherein each of the electrodes of the 96 electrode array includes a contact surface.

6. The device of claim 1 wherein the top includes top fangs.

7. The device of claim 1 wherein the base electrode array is a reusable array.

8. The device of claim 1 wherein the base electrode array is a disposable array.

9. The device of claim 1 wherein the top electrode array is a reusable array.

10. The device of claim 1 wherein the top electrode array is a disposable array.

11. The device of claim 1 wherein the base and the top are two separate structures.

12. The device of claim 1 wherein the base electrode array includes at least one wall configured to separate each electrode of the electrode array.

13. The device of claim 12 wherein the at least one wall is an insulated barrier.

14. The device of claim 1 wherein the top electrode array includes at least one wall configured to separate each electrode of the electrode array.

15. The device of claim 14 wherein the at least one wall is an insulated barrier.

16. The device of claim 1 wherein the top and base are constructed from a nonconductive material.

17. The device of claim 1 , wherein the base electrode array comprises:

an electrode base operable to conductively couple to an electroporation body;

the electrode base having a plurality of sample contact surfaces disposed thereon, the plurality of contact surfaces sized to hold an electroporation sample;

the plurality of sample contact surfaces operable to receive an electric pulse from a pulse generator; and

the plurality of sample contact surfaces sized and disposed on the electrode base to correspond with a plurality of sample contact surfaces associated with a corresponding electrode array.

18. The electrode array of claim 17 wherein the electrode base is a 96 sample base.

19. An electroporation device comprising:

a base including a base electrode array; and

a top including a top electrode array,

wherein the top includes top fangs.

Assignments (5)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 5, 2013
From: JARVIS, RICHARD A.; BYROM, MIKE W.; OVCHARENKO, DMITRIY
To: AMBION, INC.
Reel/Frame 029755/0142 →
MERGER Recorded Feb 5, 2013
From: AMBION, INC.
To: APPLERA CORPORATION
Reel/Frame 029755/0335 →
MERGER Recorded Feb 5, 2013
From: APPLERA CORPORATION
To: APPLIED BIOSYSTEMS INC.
Reel/Frame 029755/0457 →
MERGER Recorded Feb 5, 2013
From: ATOM ACQUISITION CORPORATION
To: APPLIED BIOSYSTEMS INC.
Reel/Frame 029755/0519 →
MERGER Recorded Feb 5, 2013
From: ATOM ACQUISITION, LLC AND APPLIED BIOSYSTEMS INC.
To: APPLIED BIOSYSTEMS, LLC
Reel/Frame 029755/0596 →
Continuity (4)
Continuation 12123619 · May 20, 2008
Division 11130884 · May 17, 2005
Continuation 10863102 · Jun 8, 2004
Related Publication 20100196998A1 · Aug 5, 2010