5-fluoro-uracil immunoassay
Novel conjugates of 5-fluoro-uracil and novel 5-fluoro-uracil immunogens and monoclonal antibodies generated by these immunogens which are useful in immunoassays for the quantification and monitoring of 5-fluoro-uracil in biological fluids.
1. An immunogen containing an immunogenic carrier containing a polyamine polymer linked to a compound of the formula:
wherein Y is an organic spacing group;
X is a terminal functional group involved in the binding to said polyamine polymer; and
p is an integer from 0 to 1.
2. The immunogen of claim 1 , wherein p is 0.
3. The immunogen of claim 1 , wherein p is 1.
4. The immunogen of claim 3 , wherein Y is alkylene containing from 1 to 10 carbon atoms,
wherein n and o are integers from 0 to 6, and m is an integer from 1 to 6.
5. The immunogen of claim 4 , wherein the immunogenic polymer contains one or more amino groups linked by
wherein R 4 is oxygen or sulfur.
6. The immunogen of claim 2 , wherein X is
wherein R 3 is hydrogen or taken together with its attached oxygen atom forms a reactive ester and R 4 is oxygen or sulfur.
7. The immunogen of claim 6 , wherein X is
and R 3 is hydrogen.
8. The immunogen of claim 7 , wherein X is
and R 3 forms a reactive ester.
9. The immunogen of claim 8 , wherein the ester formed is a lower alkyl ester, imidoester, or amidoester.
10. The immunogen of claim 6 , wherein the immunogenic polymer contains one or more amino groups linked by