Laser scanning microscope
View Patent ↗A laser scanning microscope that can construct a fluorescence image with high resolution even with a photodetector having a small array size is provided. A laser scanning microscope includes a line-focusing optical system that focuses laser light from a laser light source in the form of a line; a DMD having a plurality of movable micromirrors that are arrayed in the same direction of the line and reflect the focused laser light; a galvanometer mirror that scans the laser light reflected by the DMD; an irradiation optical system that irradiates a sample with the scanned laser light; a photodetector having a plurality of channels that detect light from the sample and are arrayed in a single line; and a controller that controls the DMD so as to cause the light reflected by the different movable micromirrors to enter the respective channels, and that sequentially switches between the micromirrors that reflect the light that is to enter the channels so that the light from different positions on the sample enters the channels.
1. A laser scanning microscope comprising:
a laser light source that emits laser light;
a line-focusing optical system that focuses the laser light from the laser light source in the form of a line;
a micro-element array including a plurality of micro-elements that are arrayed in the same direction of the line and reflect or transmit the laser light focused by the line-focusing optical system;
a scanner that scans the laser light reflected or transmitted by the micro-element array in a orthogonal direction of the line;
an irradiation optical system that irradiates a sample with the laser light scanned by the scanner;
a photodetector disposed at a conjugate position with respect to the micro-element array and having a plurality of channels that detect light from the sample and that are arrayed in a single line at a pitch larger than that of the micro-elements of the micro-element array; and
a controller that controls the micro-element array so as to cause the light reflected or transmitted by the different micro-elements to enter the respective channels, and that sequentially switches between the micro-elements that reflect or transmit the light that is to enter the channels so that the light from different positions on the sample enters the channels.
2. The laser scanning microscope according to claim 1 , further comprising a second scanner that scans the light from the sample in a direction extending in the same direction of the line on the sample.
3. The laser scanning microscope according to claim 1 , further comprising a dispersing element that splits the light from the sample into spectral components, arranges the spectral components in the direction orthogonal direction of the line, and emits the spectral components,
wherein multiple arrays of the photodetectors are provided in the direction orthogonal direction of the line and detect the spectral components split by the dispersing element.
4. The laser scanning microscope according to claim 1 , wherein when one focal spot is to be generated by the plurality of micro-elements, the depth of focus of at least one of the line-focusing optical system and a detection optical system that focuses light from the micro-element array onto the photodetector is set greater than an optical-path difference occurring between the micro-elements that generate the one focal spot.