Patient sample classification based upon low angle light scattering
View Patent ↗An apparatus for classifying a liquid patient sample includes at least one sample container having a quantity of a sample that is aggressively acted upon so as to create a flow field. A measurement mechanism includes at least one low angle light emitter aligned with a measurement window of the at least one sample container and a detector oppositely disposed relative to the measurement window. Measurement of the scattered light detects particle characteristics of a moving flow field from the sample to determine, for example, the amount of agglutination of the sample so as to perform blood typing or other classifications without spatial separation.
1. A method of classifying a patient sample, said method comprising the steps of:
providing a patient sample and at least one agglutination reagent into the confines of a sample container, said patient sample being disposed in an upper portion of said sample container and said at least one agglutination reagent being disposed in a lower portion of said sample container, said upper and lower portions separated by a restriction orifice;
moving the patient sample and at least one reagent within the sample container under centrifugation such said sample and said at least one reagent are sufficiently mixed so as to produce an agglutination reaction, including the step of providing at least one receiving fluid in said container having a specific viscosity and specific gravity to effectively exclude the sample prior to centrifugation thereof, but which allows particles within said sample to move therethrough when centrifugal force is applied while excluding small molecules and controlling the rate at which the material is moved within the fluid wherein only cells are subsequently moved as a flow field through the restriction orifice of said container and past an intermediately disposed measurement window of the sample container;
scanning a monochromatic light beam into the measurement window of the sample container and probing the moving flow field with the light beam of a low angle particle measurement system in which agglutinated cells of said sample in said moving flow field scatter light based on the presence of said cells and the sizing thereof; and
detecting at a light detector of the measurement system, the size and amount of said cells (both free cells and agglutinated cells) of said moving flow field based on the detection of scattered light based on the volume scattering function (VSF) of said light which is caused by the presence of said agglutinated cells in said moving flow field, said detector including a predetermined number of coaxially arranged detector rings which are arranged opposite said light beam in relation to said measurement window, each said detector ring being disposed to receive light scattered at a predetermined scattering angle, said coaxially arranged detector rings being defined to receive light scattered over a range of about 0.1 to about 20 degrees, wherein unscattered light is focused by at least one lens onto the center of said detector and in which said probing and detecting steps are performed prior to endpoint of said reaction.
2. A method as recited in claim 1 , wherein said patient sample is a blood sample.
3. A method as recited in claim 1 , wherein said sample container includes at least one chamber having a smaller inside diameter than an adjacent chamber of said container through which the mixture is moved.
4. A method as recited in claim 1 , including the step of determining the size distribution of agglutinated cells in said moving flow field.
5. A method as recited in claim 1 , wherein said detection step includes the step of blood typing based on the detected characteristics of said agglutinated cells.