Expression of Human Serum Albumin in Plastids
Human Serum Albumin (HSA) or an HSA fusion protein is expressed in plant plastids. A plastid transformation vector is made which contains an expression cassette that contains regulatory sequences, the coding region for HSA or an HSA fusion protein and a selectable marker coding sequence. The vector is used to transform a plant where the plant expresses the HSA or HSA fusion protein. HSA is isolated and purified from the plant. A preferred plant is tobacco.
1 . Human Serum Albumin (HSA) made by expressing an HSA gene in a plant plastid.
2 . A plant plastid engineered to stably produce a protective, heterologous vaccine antigen, said plastid comprising a plastid genome comprising as operably linked components one regulatory sequence and a DNA sequence encoding for said protective, heterologous vaccine antigen.
3 . The plant plastid of claim 2 , wherein said protective, heterologous vaccine antigen is bacterial.
4 . A plant comprising a plurality of plastids of claim 2 , wherein said plant thereby produces said protective, heterologous vaccine antigen and wherein said plant is edible.