Detection of antibody that binds to water soluble polymer-modified polypeptides
View Patent ↗The present invention provides analytical methods for detecting anti-polymer antibody in an individual. The methods involve contacting a sample from the individual with a water soluble polymer-modified carrier and detecting binding of antibody to the water soluble polymer on the water soluble polymer-modified carrier wherein binding is indicative of the presence of antibody to the water polymer-modified polypeptide. Antibody may be detected to water soluble polymers such as polyethylene glycol, polysialic acid, dextran, hydroxyalkyl starch, or hydroxyethyl starch. When antibody to the water soluble polymer polyethylene glycol is to be detected, the carrier is modified with a non-linear polyethylene glycol derivative.
1. A method of detecting an antibody to a polyethylene glycol (PEG)-modified polypeptide in a sample suspected of containing the antibody to the PEG-modified polypeptide, the method comprising the steps of:
a) contacting the sample with a water soluble polymer-modified carrier and
b) detecting binding of the antibody to the water soluble polymer on the water soluble polymer-modified carrier,
wherein the water soluble polymer-modified carrier comprises water soluble polymer-modified beads, wherein the water soluble polymer is a non-linear PEG polymer, and wherein binding is indicative of the presence of the antibody to the PEG-modified polypeptide.
2. The method of claim 1 wherein the polypeptide is a blood coagulation protein.
3. The method of claim 1 or 2 wherein the non-linear PEG polymer has a glycerol backbone with 1, 2 or 1,3 substitution pattern or has a lysine backbone.
4. The method of claim 1 or 2 wherein the non-linear PEG polymer is 1,3-Bis(methoxypoly(ethylene glycol)carbamoyl)-2-propanoxy-4-succinimidyl butanoate.
5. The method of claim 1 or 2 wherein the non-linear PEG polymer is linked directly to the carrier via an amide bond.
6. The method of claim 1 or 2 wherein the non-linear PEG polymer is linked directly to the carrier via an amine group.
7. The method of claim 1 or 2 wherein the non-linear PEG polymer is linked to the carriers via a spacer molecule.
8. The method of claim 7 wherein the spacer molecule is DSS (Disuccinimidyl suberate), BS3 (Bis[Sulfosuccinimidyl]suberate), EMCS(N-[ε-Maleimidocaproyloxy]succinimide ester), sulfo-EMCS(N-[ε-Maleimidocaproyloxy]sulfosuccinimide ester, MBS (m-Maleimidobenzoyl-N-hydroxysuccinimide ester), sulfo-MBS (m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester), sulfo-KMUS (N-[κ-Maleimidoundecanoyloxy]-sulfosuccinimide ester) or KMUA (N-κ-Maleimidoundecanoic acid).
9. The method of claim 1 or 2 wherein the water soluble polymer-modified beads are polystyrene beads, silica beads, latex beads or poly(methyl methacrylate) (PMMA) beads modified with a water soluble polymer.
10. The method of claim 9 wherein the water soluble polymer-modified polystyrene beads are polystyrene beads with linear PEG attached via an ethyl ether group, and wherein the non-linear PEG polymer is linked to the linear PEG via an amide bond.
11. The method of claim 2 wherein the water soluble polymer-modified blood coagulation protein is PEGylated Factor VIII.
12. The method of claim 2 wherein the water soluble polymer-modified blood coagulation protein is PEGylated Factor IX.
13. The method of claim 2 wherein the water soluble polymer-modified blood coagulation protein is PEGylated Factor VIIa.