Inhibition-based high-throughput screen strategy for cell clones
View Patent ↗A method for screening cells with high level expression of a target protein is disclosed. The method includes introducing into a plurality of host cells a DNA construct that encodes both a target protein and an inhibitor to an endogenous selectable marker in the host cells, screening host cells harboring the DNA construct for the expression of the endogenous selectable marker, and isolating cells with reduced expression of the selectable marker. Also disclosed is a DNA construct configured to express both the target protein and the inhibitor inside the host cell.
1. A method for screening cells with high level expression of a target protein, comprising:
(a) introducing into a plurality of host cells a DNA construct that encodes both a target protein and an inhibitor to an endogenous selectable marker of the host cells, wherein the DNA construct is configured to express both the target protein and the inhibitor inside the host cell;
(b) screening host cells harboring the DNA construct for expression of the endogenous selectable marker;
(c) isolating cells exhibiting reduced expression of the selectable marker; and
(d) conducting an assay using an antibody specific for the target protein to characterize cells exhibiting enhanced expression of the target protein among cells isolated in step (c).
2. The method of claim 1 , wherein the inhibitor is selected from the group consisting of small interfering RNA (siRNA), small hairpin RNA (shRNA), microRNA (miRNA), hybrid of miRNA and shRNA, and antisense RNA.
3. The method of claim 1 , wherein the endogenous selectable marker is a fluorescent marker.
4. The method of claim 1 , wherein the isolating step comprises sorting cells with a fluorescence activated cell sorter (FACS).
5. The method of claim 1 , wherein the DNA construct further encodes a dihydrofolate reductase (DHFR) and wherein the host cells are DHFR-deficient cells.
6. A high throughput screening method for selecting transgene expressing cells, comprising:
transfecting cells expressing a fluorescent protein with a vector carrying at least one transgene and an interfering RNA that inhibits the expression of the fluorescent protein;
measuring fluorescence intensity in the transfected cells; and
isolating cells having a fluorescence intensity that is lower than the fluorescence intensity of untransfected cells.
7. The method of claim 6 , wherein the fluorescent protein is green fluorescent protein (GFP).
8. The method of claim 6 , wherein the interfering RNA is a mir-30-based shRNA.
9. The method of claim 6 , wherein the isolating step comprises sorting cells with a FACS.
10. The method of claim 6 , wherein the cells that express a fluorescent protein are dihydrofolate reductase (DHFR)-deficient CHO cells.
11. A high throughput screening method for selecting transgene expressing cells, comprising:
transfecting cells expressing a fluorescent protein with a vector carrying at least one transgene and an interfering RNA that inhibits the expression of the fluorescent protein;
measuring fluorescence intensity in the transfected cells; and
harvesting cells having a fluorescence intensity that is lower than the fluorescence intensity of untransfected cells,
wherein the at least one transgene is linked to a gene encoding DHFR by an internal ribosome entry site (IRES).
12. The method of claim 11 , wherein the fluorescent protein is green fluorescent protein (GFP).
13. The method of claim 11 , wherein the interfering RNA is a mir-30-based shRNA.
14. The method of claim 11 , wherein the harvesting step comprises sorting cells with a FACS.
15. The method of claim 11 , wherein the cells that express a fluorescent protein are dihydrofolate reductase (DHFR)-deficient CHO cells.