L-lysine-containing feed additives
View Patent ↗The invention relates to relatively light and thermally stable granulated, fermentation-broth-based animal feed additives having a high content of L-lysine and to low-loss methods for their production from broths obtained by fermentation.
1. Method for producing a granulated L-lysine-containing feed additive comprising
a) fermenting L-lysine-producing microorganism in an aqueous medium under aerobic conditions to form a fermentation broth containing biomass and L-lysine, thereby completing the fermentation,
b) determining, when desired, sulphate/L-lysine ratio (V) in the fermentation broth, where V=2×[SO 4 2− ]/[L-lysine],
c) lowering pH by adding sulphuric acid to 4.9 to 5.2,
d) setting the sulphate/L-lysine ratio to 0.85 to 1.2 by adding ammonium sulphate or sulphuric acid, if necessary, and
e) granulating the mixture resulting from steps c) and d) to form a product, which granular product has an L-lysine content of 10 to 70% by weight (determined as lysine base, based on the total amount) and a pH of 3.5 to 5.1, measured as a suspension of 10% strength.
2. Method according to claim 1 , in which the broth is concentrated by removal of water between steps c) and d).
3. Method according to claim 1 , in which, before step e) adding ammonium-, earthalkali- or an alkali metal hydrogensulphite or mixtures thereof in an amount of 0.01 to 0.5% by weight, based on fermentation broth.
4. Method according to claim 1 , in which, after completion of step a) partially or completely removing the biomass formed during the fermentation.
5. Method according to claim 1 , in which the surface of the granular product is coated with an oil in an amount of 0.02 to 2% by weight, based on the total amount of the feed additive.
6. Method according to claim 1 , wherein the L-lysine-producing microorganism is coryneform bacteria.
7. Method according to claim 1 , wherein the L-lysine-producing microorganism is corynebacteria.
8. Method according to claim 1 , where ammonium sulphate present in the fermentation broth after the completion of step a) meets the sulphate/L-lysine ratio.