Compositions and methods for modulating oxidative burst in plant transformation
View Patent ↗A method for introducing a nucleic acid sequence into the genome of a plant cell and regenerating a transformed plant therefrom, including culturing the plant cell on at least one plant transformation media, the at least one plant transformation media comprising an effective amount of a compound or compounds which may modulate oxidative burst, such as melatonin, 6-hydroxy melatonin (a melatonin analog), N-t-butyl hydroxylamine (NtBH), thiamazole, 1,3 dimethylthiourea, Resveratrol, epicatechin, NADPH, or NADH.
1. A plant transformation media, comprising an effective amount of melatonin or an analog thereof, wherein the said melatonin or analog thereof has a positive effect on plant transformation efficiency.
2. The plant transformation media of claim 1 , wherein the amount of melatonin or an analog thereof in the plant transformation media is from about 1 μM to about 2000 μM.
3. The plant transformation media of claim 1 , wherein the amount of melatonin or an analog thereof in the plant transformation media is from about 5 μM to about 1500 μM.
4. The plant transformation media of claim 1 , wherein the amount of melatonin or an analog thereof in the plant transformation media is from about 10 μM to about 500 μM.
5. The plant transformation media of claim 1 , wherein the plant transformation media is suitable for inoculation of plant cell or plant tissue with Agrobacterium.
6. The plant transformation media of claim 1 , wherein the plant transformation media is suitable for co-cultivation of plant cell or plant tissue with Agrobacterium.
7. The plant transformation media of claim 1 , wherein the plant transformation media is suitable for the selection of transformed plant cells or tissues.
8. The plant transformation media of claim 1 , wherein the plant transformation media is suitable for regeneration of transformed plant cells or tissues into whole fertile plants.
9. The plant transformation media of claim 1 , wherein the plant is a monocotyledonous plant.
10. The plant transformation media of claim 1 , wherein the plant is a dicotyledonous plant.
11. The plant transformation media of claim 9 , wherein the plant is a tomato plant.
12. The plant transformation media of claim 9 , wherein the plant is a soybean plant.
13. The plant transformation media of claim 10 , wherein the plant is a rice plant.