Production of syringyl lignin in gymnosperms
View Patent ↗The present invention relates to a method for producing syringyl lignin in gymnosperms. The production of syringyl lignin in gymnosperms is accomplished by genetically transforming a gymnosperm genome, which does not normally contain genes which code for enzymes necessary for production of syringyl lignin, with DNA which codes for enzymes found in angiosperms associated with production of syringyl lignin. The expression of the inserted DNA is mediated using host promoter regions in the gymnosperm. In addition, genetic sequences which code for gymnosperm lignin anti-sense mRNA may be incorporated into the gymnosperm genome in order to suppress the formation of the less preferred forms of lignin in the gymnosperm such as guaiacyl lignin
1. A method for modifying the genome of a plant comprising
(a) fusing one or more DNA sequences to a promoter sequence of SEQ ID NO: 11 to form an expression cassette; and
(b) inserting the expression cassette into the plant to thereby produce a modified genome in the plant,
wherein the one or more DNA sequences are expressed in the plant.
2. The method of claim 1 , wherein the one or more DNA sequences are angiosperm DNA sequences.
3. The method of claim 1 , wherein the one or more DNA sequences are a genetic sequence which codes for an anti-sense mRNA.
4. The method of claim 3 , wherein the anti-sense mRNA is a gymnosperm genetic sequence which codes for the 4-coumarate CoA ligase (4CL) gene.
5. The method of claim 1 , wherein the one or more DNA sequences encode a 4-coumarate CoA ligase (4CL).
6. The method of claim 1 , wherein the plant is a gymnosperm.
7. The method of claim 6 , wherein the gymnosperm is Pinus taeda L.
8. The method of claim 1 , wherein the expression cassette is inserted into the plant genome by way of a transformation vector inserted into Agrobacterium.
9. The method of claim 8 , wherein the Agrobacterium is Agrobacterium tumefaciens EH101.
10. The method of claim 1 , wherein the expression cassette is inserted into the plant genome via direct DNA delivery to a target cell.
11. The method of claim 1 , wherein the expression cassette is inserted into the plant genome by micro-projectile bombardment of a plant cell.
12. The method of claim 1 , wherein the expression cassette is inserted into the plant genome by electroporation of a plant cell.
13. The method of claim 1 , wherein the expression cassette is inserted into the plant genome via silicon carbide whiskers.
14. The method of claim 1 , wherein the expression cassette is inserted into the plant genome via transformation of a protoplast.
15. The method of claim 1 further comprising inserting a selectable marker into the expression cassette.
16. The method of claim 15 , wherein the selectable marker encodes an enzyme which confers resistance to kanamycin or hygromycin B.