Biosensor, thin film electrode forming method, quantification apparatus, and quantification method
View Patent ↗A biosensor according to the present invention comprises a support 1 , a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3 a and 3 b parallel to the side of the support 1 , slits 4 a and 4 b vertical to the side of the support 1 , a working electrode 5 , a counter electrode 6 , a detecting electrode 7 , a spacer 8 which covers the working electrode 5 , the counter electrode 6 and the detecting electrode 7 on the support 1 , a rectangular cutout part 9 forming a specimen supply path, an inlet 9 a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5 , the counter electrode 6 and the detecting electrode 7 , which is exposed from the cutout part 9 of the spacer 8 , and a cover 13 which covers the spacer 8 , as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
1. A biosensor comprising:
a first support;
a second support which is formed at a position opposite to the first support;
an electrode part comprising a plurality of electrodes wherein at least one of the plurality of electrodes is formed on an entire internal surface of the first or the second support and at least two electrodes are formed by slits in a conductive layer on the other support;
a specimen supply path for introducing a sample liquid to the electrode part, wherein the specimen supply path comprises an inlet and an inner part; and
a reagent layer which is formed in the specimen supply path;
wherein each of the plurality of electrodes of the electrode part comprises a monolayer made of an electrical conductive substance, and
wherein the plurality of electrodes of the electrode part is formed in turn from the inlet to the inner part of the specimen supply path.
2. The biosensor as defined in claim 1 , wherein a spacer is provided with an approximate rectangular cutout part for forming the specimen supply path.
3. The biosensor as defined in claim 2 , wherein the thickness of the monolayer is less than 100 nm.
4. The biosensor as defined in claim 3 , wherein the thickness of the monolayer is 3 to 50 nm.
5. The biosensor as defined in any of claim 1 - 2 or 3 - 4 , wherein the reagent layer includes an enzyme.
6. The biosensor as defined in any of claim 1 - 2 or 3 - 4 , wherein the reagent layer includes an electron transfer agent.
7. The biosensor as defined in any of claim 1 - 2 or 3 - 4 , wherein the reagent layer includes a hydrophilic polymer.