Evanescent field modulation in a biosensor
The present invention provides an FTIR system comprising a first light source emitting light of a first wavelength, a sample volume with an adjacent sensor surface, a detector for detecting light reflected at said sensor surface. The sensor surface is illuminated by said first light source fulfilling the condition of total internal reflection and generating an evanescent field with a decay length within the sample volume. The system further comprises means for changing the decay length of the evanescent field and means for correlating the detected signals with the change of the decay length of the evanescent field.
1. FTIR system comprising a first light source emitting light of a first wavelength, a sample volume with an adjacent sensor surface, wherein the sensor surface is illuminated by said first light source fulfilling the condition of total internal reflection and generating an evanescent field with a decay length within the sample volume, a detector for detecting light reflected at the sensor surface, means for changing the decay length of the evanescent field, and means for correlating the detected signals with the change of the decay length of the evanescent field.
2. System according to claim 1 , wherein the means for changing the decay length is adapted for varying the first wavelength of the first light source.
3. System according to claim 1 , further comprising a second light source emitting light of a second wavelength different from the first wavelength and optical means allowing for illuminating the sensor surface with the first and second light source.
4. System according to claim 3 , further comprising a means for switching the first and second light source on and off in anti-phase.
5. System according to claim 1 , further comprising a means for demodulating the signal detected by the detector.
6. System according to claim 5 , further comprising a means for controlling the intensities of the light beams of the first and second light sources with respect to each other.
7. System according to claim 1 , wherein the means for changing the decay length is adapted for varying the angle between the sensor surface and the light beams of the first light source.
8. Method of detecting an FTIR bio sensor signal comprising the following steps:
a) illuminating a sensor surface adjacent to a sample volume with light of a first wavelength, wherein the condition of total internal reflection is fulfilled and an evanescent field with a decay length is generated within the sample volume;
b) detecting the light reflected at the sensor surface;
c) changing the decay length of the evanescent field during steps a) and b); and
d) correlating the detected signal with the change of the decay length of the evanescent field.
9. Method according to claim 8 , wherein the decay length of the evanescent field is changed by varying the first wavelength.
10. Method according to claim 8 , further comprising the step of illuminating the sensor surface with light of a second wavelength.
11. Method according to claim 10 , wherein the sensor surface is illuminated alternately by light of the first and second wavelength.
12. Method according to claim 8 , further comprising the step of demodulating the detected signal.
13. Method according to claim 8 , wherein the decay length of the evanescent field is changed by varying the entrance angle of the illumination.