Mammalian culture media with polyamine and iron
The present invention relates to an improved cell culture additive with a content of polyamines and iron, media containing it and processes for using it for an improved cell growth, cell viability or cellular productivity.
1. A cell culture medium wherein mammalian cells are cultured, said medium containing at least one polyamine at a concentration of from 30 mg/l to 120 mg/l and at least one iron source at a concentration of from 50 mg/l to 900 mg/l, wherein the polyamine is one or more of the compounds or salts thereof or hydrated or dehydrated forms thereof selected from: spermidine, spermine, norspermine, norspermidine, homospermine and homospermidine.
2. The cell culture medium according to claim 1 , wherein the iron source contains at least one of Fe (II) ions and Fe (III) ions.
3. The cell culture medium according to claim 1 , wherein the iron source is one or more of the compounds or salts thereof or hydrated or dehydrated forms thereof selected from: iron (III) phosphate, iron (III) pyrophosphate, iron (III) nitrate, iron (II) sulphate, iron (III) chloride, iron (II) lactate, iron (III) citrate, ammonium iron (III) citrate, iron-dextran and ethylenediaminetetraacetic acid ferric sodium salt.
4. The cell culture medium according to claim 1 , wherein the polyamine is spermine.
5. The culture medium according to claim 1 , which contains at least one compound selected from: aurin tricarboxylic acid (ATA), dichloro acetic acid (DCA), succinic acid, salts thereof and derivatives thereof.
6. The cell culture medium according to claim 1 , which is serum free.
7. The cell culture medium according to claim 1 , which is free of glutamine.
8. The cell culture medium according to claim 1 , which is protein free.
9. The cell culture medium according to claim 1 , which is provided in form of a kit.
10. A process for preparing a cell culture medium wherein mammalian cells are cultured according to claim 1 , said cell culture medium containing at least one polyamine at a concentration of from 30 mg/L to 120 mg/L and at least one iron source at a concentration of from 50 mg/L to 900mg/L, wherein the polyamine is one or more selected from the compounds or salts thereof or hydrated or dehydrated forms thereof selected from: spermidine, spermine,norspermine, norspermidine, homospermine, and homospermidine, wherein a culture additive is added to a liquid medium, wherein the culture additive contains at least one polyamine at a concentration of at least 30 mg/1 and at least one iron source at a concentration of at least 50 mg/1, wherein the polyamine is one or more of the compounds or salts thereof or hydrated or dehydrated forms thereof selected from: spermidine, spermine,norspermine, norspermidine, homospermine, and homospermidine.
11. The process according to claim 10 , wherein the liquid culture medium is a conventional culture medium containing an aqueous solvent, a carbon source and a nitrogen source.
12. A cell culture medium prepared according to the process of claim 10 .
13. A process for culturing cells in a culture medium, wherein
a) the cells and the culture medium according to claim 1 are provided, and
b) the cells are cultivated in said culture medium under conditions appropriate for maintenance of said cells in said culture.
14. The process according to claim 13 , wherein the process comprises producing at least one protein from said cells.
15. A process for culturing cells in a culture medium, wherein
a) the cells and a culture medium are provided; and b) the cells are cultivated in said culture medium under conditions appropriate for maintenance of said cells in said culture medium, and c)at least one polyamine source is added to a concentration of 30 mg/L to 120 mg/L and at least one iron source is added to a concentration of from 50 mg/L to 900mg/L, wherein the polyamine is one or more selected from the compounds or salts thereof or hydrated or dehydrated forms thereof selected from: spermidine, spermine,norspermine, norspermidine, homospermine, and homospermidine,
wherein the polyamine source and iron source are added to the culture medium before inoculating said culture medium with cells or after inoculating said first culture medium with cells or during the cultivation.