DIG-5 insecticidal Cry toxins
View Patent ↗DIG-5 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
1. An isolated polypeptide comprising a core toxin segment selected from the group consisting of
(a) a polypeptide comprising the amino acid sequence of residues 114 to 655 of SEQ ID NO:2;
(b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of residues 114 to 655 of SEQ ID NO:2; and
(c) a polypeptide comprising the amino acid sequence of residues 114to 655 of SEQ ID NO:2 with up to 20 amino acid substitutions, deletions, or modifications that do not adversely affect expression or activity of the toxin;
or an insecticidally active fragment thereof.
2. The isolated polypeptide of claim 1 comprising a core toxin segment selected from the group consisting of
(a) a polypeptide comprising the amino acid sequence of residues 1 to 655 of SEQ ID NO:2 ;
(b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of residues 1 to 655 of SEQ ID NO:2; and
(c) a polypeptide comprising the amino acid sequence of residues 1 to 655 of SEQ ID NO:2 with up to 20 amino acid substitutions, deletions, or modifications that do not adversely affect expression or activity of the toxin;
or an insecticidally active fragment thereof.
3. A plant comprising the polypeptide of claim
4. A plant comprising the polypeptide of claim 2 .
5. A method for controlling a pest population, said method comprising contacting said population with a pesticidally effective amount of the polypeptide of claim 1 .
6. An isolated polynucleotide that encodes the polypeptide of claim 1 .
7. An isolated polynucleotide that encodes the polypeptide of claim 2 .
8. The isolated polynucleotide of claim 6 , wherein said polynucleotide comprises SEQ ID NO:1 or SEQ ID NO:3.
9. The polypeptide of claim 1 , said polypeptide comprising SEQ ID NO:2 or SEQ ID NO:5.
10. A DNA construct comprising the polynucleotide of claim 6 operably linked to a promoter that is not isolated from Bacillus thuringiensis and is capable of driving expression in a plant.
11. A transgenic plant that comprises the DNA construct of claim 10 stably incorporated into its genome.
12. A method for protecting a plant from a pest, said method comprising introducing into said plant the construct of claim 10 .
13. The polypeptide of claim 1 or claim 2 having activity against corn rootworm.